Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Open Vet J. 2024 Jan;14(1):292-303. doi: 10.5455/OVJ.2024.v14.i1.26. Epub 2024 Jan 31.
Paracetamol (PCM) overdosing induces hepatotoxicity, which can result in death if the dose is high enough and the patients are not given N-acetyl cysteine. Berberine (BBR) has a variety of biological proprieties including anti-inflammatory and antioxidant activities.
Assessment of the potential effect of BBR and selenium when used alone or together on the PCM-induced acute hepatic toxicity in rats.
This research involved 40 clinically healthy mature adult male albino rats, their weights ranged from 150 to 200 g and housed in standard conditions. Our study involved evaluating the potential effect of BBR and selenium when used alone or together on the PCM-induced acute hepatic toxicity via estimation of the liver function tests, determination of the antioxidant enzyme activities, lipid peroxidation markers, immune-modulatory effects, liver histopathological, and immunohistochemical studies.
Co-treatment of BBR (150 mg/kg BW) with selenium (5 mg/kg BW) showed significant improvement in the liver function parameters, the antioxidant enzyme activities, reduction in the nitric oxide (NO), lysozyme, malondialdehyde (MDA), TNF-α, and TGF-β1 levels, and marked elevation in the IgM levels.
Altogether, BBR, selenium, or both augment antioxidant activity and alleviate PCM-induced hepatic toxicity.
对乙酰氨基酚（PCM）过量会引起肝毒性，如果剂量足够高且未给予 N-乙酰半胱氨酸，患者可能会死亡。小檗碱（BBR）具有多种生物学特性，包括抗炎和抗氧化活性。
评估 BBR 和硒单独或联合使用对 PCM 诱导的大鼠急性肝毒性的潜在影响。
本研究涉及 40 只临床健康成熟雄性白化大鼠，体重在 150 到 200 克之间，在标准条件下饲养。我们的研究通过评估 BBR 和硒单独或联合使用对 PCM 诱导的急性肝毒性的潜在影响，评估了 BBR 和硒单独或联合使用对 PCM 诱导的急性肝毒性的潜在影响，包括肝功能测试、抗氧化酶活性、脂质过氧化标志物、免疫调节作用、肝组织病理学和免疫组织化学研究。
BBR（150mg/kg BW）与硒（5mg/kg BW）联合治疗显示肝功能参数、抗氧化酶活性显著改善，一氧化氮（NO）、溶菌酶、丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）水平降低，IgM 水平显著升高。
总之，BBR、硒或两者都能增强抗氧化活性，减轻 PCM 诱导的肝毒性。
