Department of Food Hygiene, Agriculture Research Centre (ARC), Animal Health Research Institute (AHRI), Zagazig, Egypt.
Department of Botany and Microbiology, Faculty of Science, Arish University, Al-Arish, Egypt.
Open Vet J. 2024 Jan;14(1):389-397. doi: 10.5455/OVJ.2024.v14.i1.35. Epub 2024 Jan 31.
Minced meat is a valuable source of nutrients, but it is vulnerable to contamination by microorganisms commonly present in the environment. In addition, there is a risk of adulteration with cheaper meat sources, which can be harmful to consumers.
It is crucial to identify meat adulteration with distinct microbiological analysis for legal, economic, religious, and public health purposes.
A total of 100 minced meat samples were collected from several markets in Sharkia Governorate, Egypt. These samples were then subjected to bacteriological testing and an advanced multiplex PCR method. This method enables the detection of bovine, equine, porcine, and dog species in meat samples with just one step.
The adulterated samples had a higher total bacterial count and pH values compared to pure bovine meat. These differences in bacterial count and pH values were statistically significant, with values of 0.843 (log) and 0.233, respectively. The frequency of occurrence was 13%, and the O111 serotype was predominant in the adulterated samples. and were isolated with prevalence rates of 3% and 29%, respectively. Besides, the SYBR-green multiplex real-time PCR assay used in this study detected adulteration with dog, equine, and porcine meats in the examined samples at rates of 9%, 5%, and 4%, respectively.
This method provides a sensitive and specific approach to detect issues related to well-being and safety.
肉末是一种有价值的营养来源，但它容易受到环境中常见微生物的污染。此外，还存在与更便宜的肉类来源掺假的风险，这对消费者是有害的。
出于法律、经济、宗教和公共卫生的目的，必须通过独特的微生物分析来识别肉类掺假。
从埃及 Sharkia 省的几个市场收集了 100 个肉末样本。然后对这些样本进行细菌学测试和先进的多重 PCR 方法检测。这种方法可以在一步检测中检测肉类样本中的牛、马、猪和狗种。
与纯牛肉相比，掺假样本的总细菌计数和 pH 值更高。这些细菌计数和 pH 值的差异具有统计学意义，分别为 0.843(log)和 0.233。发生率为 13%，且掺假样本中以 O111 血清型为主。 和 的检出率分别为 3%和 29%。此外，本研究中使用的 SYBR 绿色多重实时 PCR 检测法在被检查的样本中检测到狗、马和猪肉掺假的比例分别为 9%、5%和 4%。
该方法为检测与福祉和安全相关的问题提供了一种敏感和特异的方法。
