Tao Zhuo, Li Xin, Yu Hongzhi, Wu Junping, Wen Ying, Liu Tianjun
Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.
Department of Pathology, Tianjin Key Laboratory of Lung Regenerative Medicine, Haihe Hospital, Tianjin 300350, China.
ACS Pharmacol Transl Sci. 2024 Mar 5;7(4):1101-1113. doi: 10.1021/acsptsci.3c00392. eCollection 2024 Apr 12.
is a Gram-negative bacterium that induces acute lung injury (ALI) and inflammation in humans, necessitating immediate hospitalization and treatment. At present, the clinical treatment is largely dependent on hormones or antibiotics but is associated with drawbacks posed by the lack of eradication of the bacterium upon treatment and drug resistance. Therefore, there is an urgent need for novel and effective treatments. The current study investigated the treatment of -induced ALI using a photosensitizer LD4 in conjunction with photodynamic therapy (PDT). The water content in the lungs (corresponding to edema) of a rat model of pneumonia induced by was reduced upon treatment with LD4-PDT. The counts of leukocyte, lymphocyte, and polymorphonuclear leukocyte in the blood were determined in the rat model of pneumonia, as were the concentrations of inflammatory cytokines (estimated using an enzyme-linked immunosorbent assay). The LD4-PDT treatment prominently reduced the levels of interleukin (IL)-6, IL-10, tumor necrosis factor-α, superoxide dismutase, and immune cells. Results suggest that LD4-PDT considerably alleviates the inflammation and oxidative stress caused by in the rat model of pneumonia. Furthermore, it could effectively improve the survival rate in the rat model of -induced pneumonia and ameliorate histological changes while protecting the integrity of the pulmonary epithelial cells. These results highlight the potential application of LD4 as a photosensitizer for treating acute pneumonia induced by .
是一种革兰氏阴性细菌，可在人类中引发急性肺损伤（ALI）和炎症，需要立即住院治疗。目前，临床治疗很大程度上依赖于激素或抗生素，但存在治疗后细菌未被根除以及耐药性带来的缺点。因此，迫切需要新颖有效的治疗方法。当前研究使用光敏剂LD4结合光动力疗法（PDT）来研究对诱导的ALI的治疗。在用LD4-PDT治疗后，由诱导的肺炎大鼠模型肺中的含水量（对应于水肿）降低。在肺炎大鼠模型中测定血液中的白细胞、淋巴细胞和多形核白细胞计数，以及炎症细胞因子的浓度（使用酶联免疫吸附测定法估计）。LD4-PDT治疗显著降低了白细胞介素（IL）-6、IL-10、肿瘤坏死因子-α、超氧化物歧化酶和免疫细胞的水平。结果表明，LD4-PDT可显著减轻肺炎大鼠模型中由引起的炎症和氧化应激。此外，它可以有效提高诱导的肺炎大鼠模型的存活率，改善组织学变化，同时保护肺上皮细胞的完整性。这些结果突出了LD4作为治疗诱导的急性肺炎的光敏剂的潜在应用。
