Önal Uğur, Saraç-Pektaş Fatma, Sağlık İmran
Department of Infectious Diseases and Clinical Microbiology, Uludağ University School of Medicine, Bursa, Türkiye.
Department of Medical Microbiology and Infection Prevention, Amsterdam University Medical Centers, Amsterdam, Netherlands.
Infect Dis Clin Microbiol. 2023 Dec 29;5(4):281-286. doi: 10.36519/idcm.2023.291. eCollection 2023 Dec.
The diagnosis of Lyme disease is becoming more common in Turkey. Nonetheless, some physicians are not aware of the diagnostic principles that should be followed when faced with a suspected patient and could use tests that are not recommended, such as darkfield microscopy. Dark field microscopy is a diagnostic technique to visualize the spirochetes that cause Lyme disease; however, it is not recommended for the diagnosis of Lyme disease. One of the main limitations of dark field microscopy is its low sensitivity. Another limitation is its high false-positivity rate, as other microorganisms and cellular debris can be mistaken for spirochetes, leading to a misdiagnosis thatmay result in unnecessary treatment. Therefore, this study aimed to review the literature on the role of dark field microscopy as a diagnostic method for Lyme disease and inform physicians about recommended approaches in line with the recommendations of national or international guidelines. An electronic search of Pubmed, Scopus, and Web of Science was performed using the following medical subject headings (MeSH) search terms: Lyme borreliosis, Lyme disease, , diagnosis, and microscopy. With this narrative review, we aimed to inform physicians better and improve patient care for patients with suspected Lyme disease.
莱姆病的诊断在土耳其越来越普遍。尽管如此，一些医生并不了解面对疑似患者时应遵循的诊断原则，可能会使用不推荐的检测方法，如暗视野显微镜检查。暗视野显微镜检查是一种用于观察引起莱姆病的螺旋体的诊断技术；然而，它并不推荐用于莱姆病的诊断。暗视野显微镜检查的主要局限性之一是其灵敏度低。另一个局限性是其假阳性率高，因为其他微生物和细胞碎片可能被误认为是螺旋体，从而导致误诊，可能会导致不必要的治疗。因此，本研究旨在回顾关于暗视野显微镜检查作为莱姆病诊断方法作用的文献，并根据国家或国际指南的建议告知医生推荐的方法。使用以下医学主题词（MeSH）检索词对PubMed、Scopus和Web of Science进行了电子检索：莱姆疏螺旋体病、莱姆病、诊断和显微镜检查。通过本叙述性综述，我们旨在更好地告知医生，并改善对疑似莱姆病患者的护理。
