College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Edu-cation, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, P. R. China.
Laoshan Laboratory, 168 Wenhai Middle Rd, Aoshanwei Jimo, Qingdao ,Shandong266237, P. R. China.
Anal Chem. 2024 Apr 30;96(17):6812-6818. doi: 10.1021/acs.analchem.4c00794. Epub 2024 Apr 18.
Among the primary threats to human health worldwide, nonsmall cell lung cancer (NSCLC) remains a significant factor and is a leading cause of cancer-related deaths. Due to subtle early symptoms, NSCLC patients are diagnosed at advanced stages, resulting in low survival rates. Herein, novel Au-Se bond nanoprobes (NPs) designed for the specific detection of Calpain-2 (CAPN2) and Human Neutrophil Elastase (HNE), pivotal biomarkers in NSCLC, were developed. The NPs demonstrated exceptional specificity and sensitivity toward CAPN2 and HNE, enabling dual-color fluorescence imaging to distinguish between NSCLC cells and normal lung cells effectively. The NPs' performance was consistent across a wide pH range (6.2 to 8.0), and it exhibited remarkable resistance to biological thiol interference, indicating its robustness in complex physiological environments. These findings suggest the nanoprobe is a promising tool for early NSCLC diagnosis, offering a novel approach for enhancing the accuracy of cancer detection.
在全球范围内对人类健康的主要威胁中，非小细胞肺癌（NSCLC）仍然是一个重要因素，也是癌症相关死亡的主要原因。由于早期症状不明显，NSCLC 患者在晚期被诊断出来，导致生存率低。在此，设计了用于特异性检测非小细胞肺癌中关键生物标志物钙蛋白酶-2（CAPN2）和人中性粒细胞弹性蛋白酶（HNE）的新型 Au-Se 键纳米探针（NPs）。这些 NPs 对 CAPN2 和 HNE 表现出出色的特异性和灵敏度，能够进行双色荧光成像，有效地将 NSCLC 细胞与正常肺细胞区分开来。该 NPs 在很宽的 pH 值范围（6.2 至 8.0）内都具有一致的性能，并且对生物硫醇干扰具有显著的抗性，表明其在复杂的生理环境中具有强大的稳定性。这些发现表明该纳米探针是一种有前途的早期 NSCLC 诊断工具，为提高癌症检测的准确性提供了一种新方法。
