Wang Yunpeng, Chen Shiyi, Liu Yifan, Zhang Shufei, Jin Xun, Zheng Sixu, Li Jiasheng, Peng Ying, Zhang Kun, Zhang Chi, Liu Bingjian
National engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, No.1, Haida South Road, Zhoushan, 316022, Zhejiang, People's Republic of China.
Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou, 510300, Guangdong, China.
Biochem Genet. 2025 Apr;63(2):1901-1923. doi: 10.1007/s10528-024-10793-7. Epub 2024 Apr 18.
The family Sisoridae is one of the largest and most diverse Asiatic catfish families, with most species occurring in the water systems of the Qinhai-Tibetan Plateau and East Himalayas. At present, the phylogenetic relationship of the Sisoridae is relatively chaotic. In this study, the mitochondrial genomes (mitogenomes) of three species Creteuchiloglanis kamengensis, Glaridoglanis andersonii, and Exostoma sp. were systematically investigated, the phylogenetic relationships of the family were reconstructed and to determine the phylogenetic position of Exostoma sp. within Sisoridae. The lengths of the mitogenomes' sequences of C. kamengensis, G. andersonii, and Exostoma sp. were 16,589 bp, 16,531 bp, and 16,529 bp, respectively. They all contained one identical control region (D-loop), two ribosomal RNAs (rRNAs), 13 protein-coding genes (PCGs) and 22 transfer RNA (tRNA) genes. We applied two approaches, Bayesian Inference (BI) and Maximum Likelihood (ML), to construct phylogenetic trees. Our findings revealed that the topological structure of both ML and BI trees exhibited significant congruence. Specifically, the phylogenetic tree strongly supports the monophyly of Sisorinae and Glyptosternoids and provides new molecular biological data to support the reconstruction of phylogenetic relationships with Sisoridae. This study is of great scientific value for phylogenetic and genetic variation studies of the Sisoridae.
吸口鲶科是亚洲最大且最多样化的鲶鱼科之一，大多数物种分布于青藏高原和东喜马拉雅山脉的水系中。目前，吸口鲶科的系统发育关系相对混乱。在本研究中，对卡门异齿吸口鲶、安氏纹胸鮡和外口鲶属某物种的线粒体基因组进行了系统研究，重建了该科的系统发育关系，并确定了外口鲶属某物种在吸口鲶科中的系统发育位置。卡门异齿吸口鲶、安氏纹胸鮡和外口鲶属某物种的线粒体基因组序列长度分别为16,589 bp、16,531 bp和16,529 bp。它们均包含一个相同的控制区（D环）、两个核糖体RNA（rRNA）、13个蛋白质编码基因（PCG）和22个转运RNA（tRNA）基因。我们采用贝叶斯推断（BI）和最大似然法（ML）两种方法构建系统发育树。我们的研究结果表明，ML树和BI树的拓扑结构显示出显著的一致性。具体而言，系统发育树有力地支持了吸口鲶亚科和纹胸鮡类的单系性，并提供了新的分子生物学数据来支持吸口鲶科系统发育关系的重建。本研究对吸口鲶科的系统发育和遗传变异研究具有重要的科学价值。
