School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
Shandong Water Conservancy Vocational College, Rizhao 276826, P. R. China.
Anal Chem. 2024 Apr 30;96(17):6659-6665. doi: 10.1021/acs.analchem.4c00048. Epub 2024 Apr 18.
The enhancement of sensitivity in biological analysis detection can reduce the probability of false positives of the biosensor. In this work, a novel self-on controlled-release electrochemiluminescence (CRE) biosensor was designed by multiple signal amplification and framework-enhanced stability strategies. As a result, the changes of the ECL signal were enhanced before and after the controlled-release process, achieving sensitive detection of prostate-specific antigen (PSA). Specifically, for one thing, FeO@CeO-NH with two paths for enhancing the generation of coreactant radicals was used as the coreaction accelerator to boost ECL performance. For another, due to the framework stability, zeolitic imidazolate framework-8-NH (ZIF-8-NH) was combined with luminol to make the ECL signal more stable. Based on these strategies, the constructed CRE biosensor showed a strong self-on effect in the presence of PSA and high sensitivity in a series of tests. The detection range and limit of detection (LOD) were 5 fg/mL to 10 ng/mL and 2.8 fg/mL (S/N = 3), respectively, providing a feasible approach for clinical detection of PSA.
生物分析检测灵敏度的提高可以降低生物传感器假阳性的概率。在这项工作中，通过多重信号放大和框架增强稳定性策略，设计了一种新型的自控释放电化学发光（CRE）生物传感器。结果，在控制释放过程前后，ECL 信号的变化得到增强，实现了对前列腺特异性抗原（PSA）的灵敏检测。具体而言，一方面，使用具有两种增强反应剂自由基生成途径的 FeO@CeO-NH 作为共反应加速剂来提升 ECL 性能。另一方面，由于框架稳定性，沸石咪唑酯骨架-8-NH（ZIF-8-NH）与鲁米诺结合使 ECL 信号更加稳定。基于这些策略，构建的 CRE 生物传感器在存在 PSA 时表现出强烈的自启动效应，并且在一系列测试中具有高灵敏度。检测范围和检测限（LOD）分别为 5 fg/mL 至 10 ng/mL 和 2.8 fg/mL（S/N = 3），为 PSA 的临床检测提供了一种可行的方法。
