Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine & Pharmacy, Komenského 73, 040 01, Košice, Slovakia.
Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Science, Watsonova 1935/47, 040 01, Košice, Slovakia.
Arch Biochem Biophys. 2024 Jun;756:110001. doi: 10.1016/j.abb.2024.110001. Epub 2024 Apr 16.
The use of insecticides presents a risk to the environment because they can accumulate in the water, soil, air, and organisms, endangering human and animal health. It is therefore essential to investigate the effects of different groups of insecticides on individual biomacromolecules such as DNA. We studied fipronil, which belongs to the group of phenylpyrazole insecticides. The interaction of fipronil with calf thymus DNA was investigated using spectroscopic methods (absorption and fluorescence spectroscopy) complemented with infrared spectroscopy and viscosity measurement. Fluorescence emission spectroscopy showed the formation of a fipronil/DNA complex with a combined static and dynamic type of quenching. The binding constant was 4.15 × 10 L/mol. Viscosity changes were recorded to confirm/disconfirm the intercalation mode of interaction. A slight change in DNA viscosity in the presence of fipronil was observed. The phenylpyrazole insecticide does not cause significant conformational changes in DNA structure or increase of its chain length. We hypothesize that fipronil is incorporated into the minor groove of the DNA macromolecule via hydrogen interactions as indicated by FT-IR and CD measurements.
杀虫剂的使用会对环境造成危害，因为它们会在水、土壤、空气和生物体内积累，危及人类和动物的健康。因此，研究不同类别的杀虫剂对 DNA 等生物大分子的影响至关重要。我们研究了属于苯并吡唑类杀虫剂的氟虫腈。利用光谱法（吸收和荧光光谱法）结合红外光谱法和粘度测量法研究了氟虫腈与小牛胸腺 DNA 的相互作用。荧光发射光谱表明形成了氟虫腈/DNA 复合物，具有静态和动态混合类型的猝灭。结合常数为 4.15×10^L/mol。记录粘度变化以确认/否定相互作用的嵌入模式。在存在氟虫腈的情况下，观察到 DNA 粘度略有变化。苯并吡唑类杀虫剂不会导致 DNA 结构的显著构象变化或链长增加。我们假设氟虫腈通过氢键相互作用嵌入 DNA 大分子的小沟中，这一点通过 FT-IR 和 CD 测量得到了证实。
