Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou, 535011, China.
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou, 535011, China.
Fish Shellfish Immunol. 2024 Jun;149:109566. doi: 10.1016/j.fsi.2024.109566. Epub 2024 Apr 16.
Fish rely on innate immune system for immunity, and nucleotide-binding oligomerization domain-like receptors (NLRs) are a vital group of receptor for recognition. In the present study, NOD1 gene was cloned and characterized from golden pompano Trachinotus ovatus, a commercially important aquaculture fish species. The ORF of T. ovatus NOD1 was 2820 bp long, encoding 939 amino acid residues with a highly conserved domains containing CARD-NACHT-LRRs. Phylogenetic analysis revealed that the T. ovatus NOD1 clustered with those of fish and separated from those of birds and mammals. T. ovatus NOD1 has wide tissue distribution with the highest expression in gills. Bacterial challenges (Streptococcus agalactiae and Vibrio alginolyticus) significantly up-regulated the expression of NOD1 with different response time. The results of T. ovatus NOD1 ligand recognition and signaling pathway analysis revealed that T. ovatus NOD1 could recognize iE-DAP at the concentration of ≧ 100 ng/mL and able to activate NF-κB signaling pathway. This study confirmed that NOD1 play a crucial role in the innate immunity of T. ovatus. The findings of this study improve our understanding on the immune function of NOD1 in teleost, especially T. ovatus.
鱼类依赖先天免疫系统来获得免疫力，核苷酸结合寡聚化结构域样受体（NLRs）是识别的重要受体群。本研究从金鲳 Trachinotus ovatus 克隆和表征了 NOD1 基因，金鲳是一种具有商业重要性的水产养殖鱼类。T. ovatus NOD1 的 ORF 长 2820bp，编码 939 个氨基酸残基，具有高度保守的结构域，包含 CARD-NACHT-LRRs。系统进化分析表明，T. ovatus NOD1 与鱼类的 NOD1 聚类，并与鸟类和哺乳动物的 NOD1 分离。T. ovatus NOD1 具有广泛的组织分布，在鳃中的表达量最高。细菌挑战（无乳链球菌和溶藻弧菌）以不同的反应时间显著上调了 NOD1 的表达。T. ovatus NOD1 配体识别和信号通路分析的结果表明，T. ovatus NOD1 可以在 ≧ 100ng/mL 的浓度下识别 iE-DAP，并能够激活 NF-κB 信号通路。本研究证实了 NOD1 在 T. ovatus 先天免疫中起着至关重要的作用。这项研究的结果提高了我们对 NOD1 在硬骨鱼类中的免疫功能的理解，特别是在 T. ovatus 中。
