Federal University of São Carlos (UFSCar), Department of Biology (DBio-So), Laboratory of Structural and Functional Biology (LABEF), Brazil; Postgraduate Program in Biotechnology and Environmental Monitoring (PPGBMA), Center for Science and Technology for Sustainability (CCTS), UFSCar, Sorocaba, SP, Brazil.
Federal University of São Carlos (UFSCar), Department of Biology (DBio-So), Laboratory of Structural and Functional Biology (LABEF), Brazil; Postgraduate Program in Biotechnology and Environmental Monitoring (PPGBMA), Center for Science and Technology for Sustainability (CCTS), UFSCar, Sorocaba, SP, Brazil.
Environ Toxicol Pharmacol. 2024 Jun;108:104450. doi: 10.1016/j.etap.2024.104450. Epub 2024 Apr 16.
Eusocial bees are declining due to anthropogenic actions. Individuals can be exposed to contaminants like Cd, which have been found in pollen. Thus, we evaluated the potential genotoxicity of Cd to the hepato-nephrocitic system of Bombus atratus foraging workers exposed to environmentally safe doses of Cd (0.001 mg/kg and 0.003 mg/kg) during 72 h. To assess the genotoxicity of Cd, we conducted an alkaline comet assay. Doses of 0.001 mg/kg of Cd caused comets of levels 2 and 3, and 0.003 mg/kg of Cd induced comets of level 4, while controls exhibited regular nucleoids. Also, 0.003 mg/kg doses caused higher Damage Index, Damage Frequency, and Total Damage. Our results bring new evidence that 0.003 mg/kg Cd exerted genotoxicity to the HNS cells of B. atratus, probably affecting secondary metabolism and the detoxification capability of bees.
由于人类活动的影响，群居蜜蜂正在减少。个体可能会接触到污染物，如 Cd，这些污染物已在花粉中被发现。因此，我们评估了环境安全剂量的 Cd（0.001mg/kg 和 0.003mg/kg）对采集花粉的 B. atratus 蜂的肝-肾细胞系统的潜在遗传毒性，暴露时间为 72 小时。为了评估 Cd 的遗传毒性，我们进行了碱性彗星试验。0.001mg/kg 的 Cd 剂量导致彗星水平 2 和 3，而 0.003mg/kg 的 Cd 则诱导彗星水平 4，而对照组则显示出规则的核体。此外，0.003mg/kg 的剂量还导致更高的损伤指数、损伤频率和总损伤。我们的结果提供了新的证据，表明 0.003mg/kg 的 Cd 对 B. atratus 的 HNS 细胞具有遗传毒性，可能影响蜜蜂的次级代谢和解毒能力。
