Department of Animal Biosciences, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Hybrid Turkeys, 650 Riverbend Drive Suite C, Kitchener, ON, N2K 3S2, Canada.
Sci Rep. 2024 Apr 18;14(1):9007. doi: 10.1038/s41598-024-59309-8.
White striping (WS) is a myopathy of growing concern to the turkey industry. It is rising in prevalence and has negative consequences for consumer acceptance and the functional properties of turkey meat. The objective of this study was to conduct a genome-wide association study (GWAS) and functional analysis on WS severity. Phenotypic data consisted of white striping scored on turkey breast fillets (N = 8422) by trained observers on a 0-3 scale (none to severe). Of the phenotyped birds, 4667 genotypic records were available using a proprietary 65 K single nucleotide polymorphism (SNP) chip. The SNP effects were estimated using a linear mixed model with a 30-SNP sliding window approach used to express the percentage genetic variance explained. Positional candidate genes were those located within 50 kb of the top 1% of SNP windows explaining the most genetic variance. Of the 95 positional candidate genes, seven were further classified as functional candidate genes because of their association with both a significant gene ontology and molecular function term. The results of the GWAS emphasize the polygenic nature of the trait with no specific genomic region contributing a large portion to the overall genetic variance. Significant pathways relating to growth, muscle development, collagen formation, circulatory system development, cell response to stimulus, and cytokine production were identified. These results help to support published biological associations between WS and hypoxia and oxidative stress and provide information that may be useful for future-omics studies in understanding the biological associations with WS development in turkeys.
白条（WS）是一种日益引起火鸡产业关注的肌肉疾病。它的发病率正在上升，对消费者的接受程度和火鸡肉的功能特性产生了负面影响。本研究的目的是对 WS 严重程度进行全基因组关联研究（GWAS）和功能分析。表型数据由经过培训的观察者在 0-3 级（无到严重）对火鸡胸脯肉片上的白条进行评分（N=8422）组成。在所表型的鸟类中，有 4667 个基因型记录可通过专有的 65K 单核苷酸多态性（SNP）芯片获得。使用线性混合模型估计 SNP 效应，该模型采用 30-SNP 滑动窗口方法来表示解释的遗传方差百分比。位置候选基因是那些位于解释遗传方差最多的前 1% SNP 窗口内 50kb 以内的基因。在 95 个位置候选基因中，有 7 个被进一步归类为功能候选基因，因为它们与一个显著的基因本体和分子功能术语都有关联。GWAS 的结果强调了该性状的多基因性质，没有特定的基因组区域对整体遗传方差有很大贡献。与生长、肌肉发育、胶原蛋白形成、循环系统发育、细胞对刺激的反应和细胞因子产生相关的显著途径被确定。这些结果有助于支持 WS 与缺氧和氧化应激之间的已发表生物学关联，并提供可能有助于未来通过组学研究了解 WS 在火鸡中的发展的生物学关联的信息。
