棕色固氮菌固氮酶结构基因簇的完整核苷酸序列。
Complete nucleotide sequence of the Azotobacter vinelandii nitrogenase structural gene cluster.
作者信息
Brigle K E, Newton W E, Dean D R
出版信息
Gene. 1985;37(1-3):37-44. doi: 10.1016/0378-1119(85)90255-0.
DNA fragments coding for the structural genes for Azotobacter vinelandii nitrogenase have been isolated and sequenced. These genes, nifH, nifD and nifK, code for the iron (Fe) protein and the alpha and beta subunits of the molybdenum-iron (MoFe) protein, respectively. They are arranged in the order: promoter:nifH:nifD:nifK. There are 129 nucleotides separating nifH and nifD and 101 nucleotides separating nifD and nifK. The amino acid (aa) sequences deduced from the nucleotide sequences are discussed in relation to the prosthetic group-binding regions of the nifHDK-encoded polypeptides.
编码棕色固氮菌固氮酶结构基因的DNA片段已被分离和测序。这些基因，即nifH、nifD和nifK，分别编码铁（Fe）蛋白以及钼铁（MoFe）蛋白的α亚基和β亚基。它们按以下顺序排列：启动子:nifH:nifD:nifK。nifH和nifD之间有129个核苷酸，nifD和nifK之间有101个核苷酸。从核苷酸序列推导出来的氨基酸（aa）序列，将结合nifHDK编码的多肽的辅基结合区域进行讨论。
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