Noronha Natália Yumi, Noma Isabella Harumi Yonehara, Fernandes Ferreira Rafael, Rodrigues Guilherme da Silva, Martins Luzania Dos Santos, Watanabe Lígia Moriguchi, Pinhel Marcela Augusta de Souza, Mello Schineider Isabelle, Diani Luísa Maria, Carlos Daniela, Nonino Carla Barbosa
Department of Gynecology and Obstetrics, University Medical Center Groningen, Groningen, Netherlands.
Faculty of Medicine of Ribeirão Preto, Department of Internal Medicine, University of São Paulo, Ribeirão Preto, Brazil.
Front Nutr. 2024 Apr 4;11:1373499. doi: 10.3389/fnut.2024.1373499. eCollection 2024.
There is an emerging body of evidence that vitamin C consumption can modulate microbiota abundance and can also impact DNA methylation in the host, and this could be a link between diet, microbiota, and immune response. The objective of this study was to evaluate common CpG sites associated with both vitamin C and microbiota phyla abundance.
Six healthy women participated in this cohort study. They were divided into two groups, according to the amount of vitamin C they ingested. Ingestion was evaluated using the 24-h recall method. The Illumina 450 k BeadChip was used to evaluate DNA methylation. Singular value decomposition analyses were used to evaluate the principal components of this dataset. Associations were evaluated using the differentially methylated position function from the Champ package for R Studio.
The group with higher vitamin C (HVC) ingestion also had a higher relative abundance of Actinobacteria. There was a positive correlation between those variables ( = 0.84, = 0.01). The HVC group also had higher granulocytes, and regarding DNA methylation, there were 207 CpG sites commonly related to vitamin C ingestion and the relative abundance of Actinobacteria. From these sites, there were 13 sites hypomethylated and 103 hypermethylated. The hypomethylated targets involved the respective processes: immune function, glucose homeostasis, and general cellular metabolism. The hypermethylated sites were also enriched in immune function-related processes, and interestingly, more immune responses against pathogens were detected. These findings contribute to understanding the interaction between nutrients, microbiota, DNA methylation, and the immune response.
越来越多的证据表明，维生素C的摄入可以调节微生物群的丰度，也会影响宿主的DNA甲基化，这可能是饮食、微生物群和免疫反应之间的一个联系。本研究的目的是评估与维生素C和微生物门类丰度相关的常见CpG位点。
六名健康女性参与了这项队列研究。根据她们摄入的维生素C量，将她们分为两组。采用24小时回顾法评估摄入量。使用Illumina 450 k BeadChip评估DNA甲基化。使用奇异值分解分析来评估该数据集的主成分。使用R Studio的Champ包中的差异甲基化位置函数评估关联性。
维生素C摄入量较高（HVC）的组中放线菌的相对丰度也较高。这些变量之间存在正相关（r = 0.84，P = 0.01）。HVC组的粒细胞也较多，在DNA甲基化方面，有207个CpG位点与维生素C摄入和放线菌的相对丰度共同相关。在这些位点中，有13个位点低甲基化，103个位点高甲基化。低甲基化靶点涉及各自的过程：免疫功能、葡萄糖稳态和一般细胞代谢。高甲基化位点也富集在免疫功能相关过程中，有趣的是，检测到更多针对病原体的免疫反应。这些发现有助于理解营养素、微生物群、DNA甲基化和免疫反应之间的相互作用。
