Department of Laboratory Medicine, Zhoupu Hospital, Shanghai University of Medicine & Health Sciences, Shanghai, China.
Clinical Research Center, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Front Cell Infect Microbiol. 2024 Apr 4;14:1368450. doi: 10.3389/fcimb.2024.1368450. eCollection 2024.
To evaluate the antibacterial effect of Tanreqing (TRQ) against and its inhibition activity on bacterial biofilm formation and , and to explore the mechanism of the inhibitory effects of TRQ on biofilm formation.
An biofilm model of was established, and the impact of TRQ on biofilm formation was evaluated using crystal violet staining and scanning electron microscopy (SEM). Furthermore, the clearance effect of TRQ against in the biofilm was assessed using the viable plate counting method; q-RT PCR as used to evaluate the inhibitory effect of different concentrations of TRQ on the expression of biofilm-related genes in ; The activity of quorum sensing signal molecule AI-2 was detected by bioluminescence assay; Meanwhile, a guinea pig lung infection model of was constructed, and after treated with drugs, pathological analysis of lung tissue and determination of bacterial load in lung tissue were performed. The treatment groups included TRQ group, imipenem(IPM) group, TRQ+IPM group, and sterile saline group as the control.
The formation of biofilm was significantly inhibited by TRQ experiments. Furthermore, when combined with IPM, the clearance of in the biofilm was notably increased compared to the TRQ group and IPM group alone. q-RT PCR analysis revealed that TRQ down-regulated the expression of genes related to biofilm formation in , specifically , , , and , and also inhibited the activity of AI-2 molecules in the bacterium. experiments demonstrated that TRQ effectively treated guinea pig lung infections, resulting in reduced lung inflammation. Additionally, when combined with IPM, there was a significant reduction in the bacterial load in lung tissue.
TRQ as a potential therapeutic agent plays a great role in the treatment of infections, particularly in combination with conventional antibiotics. And TRQ can enhanced the clearance effect on the bacterium by inhibiting the biofilm formation, which provided experimental evidence in support of clinical treatment of TRQ against infections.
评价痰热清（TRQ）对 的抗菌作用及其对细菌生物膜形成的抑制活性，并探讨 TRQ 抑制 生物膜形成的机制。
建立 生物膜模型，采用结晶紫染色和扫描电子显微镜（SEM）评价 TRQ 对生物膜形成的影响。进一步采用活菌平板计数法评估 TRQ 对生物膜中 的清除作用；q-RT PCR 评估不同浓度 TRQ 对 生物膜相关基因表达的抑制作用；采用生物发光法检测群体感应信号分子 AI-2 的活性；同时构建 豚鼠肺部感染模型，给药后进行肺组织病理分析和肺组织细菌载量测定。治疗组包括 TRQ 组、亚胺培南（IPM）组、TRQ+IPM 组和无菌生理盐水组作为对照组。
TRQ 实验显著抑制 生物膜的形成。此外，与 IPM 联合使用时，与 TRQ 组和单独使用 IPM 组相比，生物膜中 的清除率明显增加。q-RT PCR 分析显示，TRQ 下调 生物膜形成相关基因的表达，特别是 、 、 、 和 ，并抑制细菌中 AI-2 分子的活性。 实验表明，TRQ 有效治疗了豚鼠肺部感染，减轻了肺部炎症。此外，与 IPM 联合使用时，肺组织中的细菌载量显著降低。
TRQ 作为一种潜在的治疗药物，在治疗 感染方面发挥着重要作用，特别是与传统抗生素联合使用时。TRQ 通过抑制 生物膜形成增强了对细菌的清除作用，为临床使用 TRQ 治疗 感染提供了实验依据。
