Department of Pathology and Histology, School of Medicine, Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, Iran.
Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Pathol Res Pract. 2024 May;257:155291. doi: 10.1016/j.prp.2024.155291. Epub 2024 Apr 4.
Because long non-coding RNAs (lncRNAs) can affect several interconnected processes, its value as a predictive marker for gastric cancer has been demonstrated. Coumarin - a natural compound known to contain some beneficial antitumor qualities - was tested for its effects on AGS gastric cancer cells. In this study, we investigated the expression level of selected cellular lncRNAs (BANCR, MALAT1 and FER1L4) and their target genes (PTEN, p-PI3K and p-AKT) in coumarin-treated AGS cell line. The expressions of the three lncRNAs: BANCR, MALAT1 and FER1L4, as well as their specified targets, PTEN, PI3K and AKT, were measured by qRT-PCR. To gauge the impact of coumarin on the AGS cells, a MTT assay was utilized. A Western blot has been employed to assess variations in PTEN, p-PI3K, and p-AKT expression. The experiment's results showed that AGS viability diminished with increasing doses of coumarin. Compared to the control cells, the cells exposed to coumarin had showed reduced levels of mRNAs which are known targets of the lncRNA BANCR. At the same time, levels of lncRNAs MALAT1 and FER1L4 within coumarin group have been higher comparing to those within control group. Additionally, the Western blot analysis revealed that the coumarin-treated cells expressed lower levels of p-PI3K, PTEN as well as p-AKT compared to control group. This information points to coumarin being a possible option in a treatment regimen for gastric cancer due to its ability to affect lncRNAs and the molecules they target.
由于长链非编码 RNA（lncRNA）可以影响多个相互关联的过程，因此它作为胃癌预测标志物的价值已经得到了证明。香豆素是一种已知具有一些有益抗肿瘤特性的天然化合物，其对 AGS 胃癌细胞的作用已被测试。在这项研究中，我们研究了选定的细胞 lncRNA（BANCR、MALAT1 和 FER1L4）及其靶基因（PTEN、p-PI3K 和 p-AKT）在香豆素处理的 AGS 细胞系中的表达水平。通过 qRT-PCR 测量了三个 lncRNA：BANCR、MALAT1 和 FER1L4 以及它们指定的靶基因 PTEN、PI3K 和 AKT 的表达。为了评估香豆素对 AGS 细胞的影响，我们使用了 MTT 测定法。Western blot 被用来评估 PTEN、p-PI3K 和 p-AKT 表达的变化。实验结果表明，AGS 细胞活力随着香豆素剂量的增加而降低。与对照组细胞相比，暴露于香豆素的细胞显示出已知 BANCR lncRNA 靶基因的 mRNA 水平降低。同时，香豆素组内的 lncRNA MALAT1 和 FER1L4 水平高于对照组。此外，Western blot 分析表明，与对照组相比，香豆素处理的细胞表达的 p-PI3K、PTEN 和 p-AKT 水平较低。这些信息表明香豆素可能是胃癌治疗方案中的一种选择，因为它能够影响 lncRNA 及其靶分子。
