Lubis Lokot Donna, Prananda Arya Tjipta, Juwita Nur Aira, Nasution Muhammad Amin, Syahputra Rony Abdi, Sumaiyah Sumaiyah, Lubis Rodiah Rahmawaty, Lubis Muhammad Fauzan, Astyka Ririn, Atiqah Jihan Firyal
Department of Histology, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia.
Department of Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, 20155, Indonesia.
Heliyon. 2024 Apr 10;10(8):e29541. doi: 10.1016/j.heliyon.2024.e29541. eCollection 2024 Apr 30.
The medicinal plant has a long history of usage in traditional medicine. Plant extracts have several interesting pharmacological effects but have some drawbacks that can be addressed via capsulation with chitosan. This work set out to do just that tally up the antioxidant effects of a polyphenol-rich extract and see how capsulation affected them. The reflux extraction and response surface methodology (RSM) were carried out to optimize the phenolic and flavonoid content of extract. Additionally, high-resolution mass spectrometry was employed to determine the secondary metabolite present in the extract. The microcapsules of extract-loaded chitosan were prepared using the ionic gelation method and characterized in terms of size, encapsulation efficiency (EE), and morphology of microcapsules. Fourier transform infrared (FTIR) was used to observe the successful production of microcapsules with a principal component analysis (PCA) approach. The antioxidant activity of microcapsules was established using the radical scavenging method. According to RSM, the highest amounts of TPC and TFC were obtained at 72.894 % ethanol, 2.031 h, and 57.384 °C. The compounds were employed from the optimized extract of including phenolics and flavonoids. The microcapsules were secured with a %EE of 43.56 ± 2.31 %. The characteristics of microcapsules were approved for the obtained product's successful synthesis according to the PCA. The microcapsules have antioxidant activity in a concentration-dependent manner ( < 0.0001). The findings of this study underscored the benefits of employing chitosan as a nanocarrier for extract, offering a promising approach to enhance plant-derived therapies.
这种药用植物在传统医学中的使用历史悠久。植物提取物具有多种有趣的药理作用，但也存在一些缺点，可通过用壳聚糖包封来解决。这项工作正是为此展开，旨在总结富含多酚的提取物的抗氧化作用，并观察包封对其的影响。采用回流提取和响应面法（RSM）来优化提取物中酚类和黄酮类化合物的含量。此外，还使用高分辨率质谱法来确定提取物中存在的次生代谢物。采用离子凝胶法制备了负载提取物的壳聚糖微胶囊，并对其尺寸、包封率（EE）和微胶囊形态进行了表征。利用傅里叶变换红外光谱（FTIR）并采用主成分分析（PCA）方法来观察微胶囊的成功制备。使用自由基清除法确定微胶囊的抗氧化活性。根据RSM，在72.894%乙醇、2.031小时和57.384°C条件下获得了最高含量的总酚（TPC）和总黄酮（TFC）。从包括酚类和黄酮类化合物的优化提取物中获取这些化合物。微胶囊的包封率为43.56±2.31%。根据PCA，微胶囊的特性表明所获得的产品成功合成。微胶囊具有浓度依赖性的抗氧化活性（P<0.0001）。本研究结果强调了将壳聚糖用作提取物纳米载体的益处，为增强植物源疗法提供了一种有前景的方法。
