Phupaboon Srisan, Matra Maharach, Prommachart Ronnachai, Totakul Pajaree, Supapong Chanadol, Wanapat Metha
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Animal Science, Faculty of Agriculture and Natural Resources, Rajamangala University of Technology, Tawan-Ok, Chonburi 20110, Thailand.
Antioxidants (Basel). 2022 Oct 25;11(11):2103. doi: 10.3390/antiox11112103.
The objective of the research was to investigate the bioactive compounds of herbal plant leaves by microencapsulation technique for future application as a feed additive. In this experiment, three herbal plant leaves, namely Cannabis sativa L., Cannabis indica L., and Mitragyna speiosa K., were comparatively investigated using different methods to extract their bioactive compounds. Two methods were used to extract the bioactive compounds: microwave extraction (water-heating transferred) and maceration extraction (methanol extracted). The results obtained using microwave extraction revealed that the total polyphenolic and flavonoid contents and antioxidant capacity were significantly higher and stronger, respectively, than those produced by the maceration extraction method (p < 0.05). Furthermore, the spray-drying technique was employed to enhance the extracted compounds by encapsulation with chitosan through ionic gelation properties. The physical characteristics of chitosan-encapsulated substrates were examined under a scanning electron microscope (SEM) and were as microparticle size (1.45 to 11.0 µm). The encapsulation efficiency of the bioactive compounds was found to be 99.7, 82.3, and 54.6% for microencapsulated M. speiosa, C. indica, and C. sativa, respectively. Therefore, microwave treatment prior to chitosan encapsulation of leaf extracts resulted in increased recovery of bioactive compound encroachment.
本研究的目的是通过微囊化技术研究草本植物叶片中的生物活性化合物，以便将来用作饲料添加剂。在本实验中，对三种草本植物叶片，即大麻、印度大麻和帽蕊木进行了比较研究，采用不同方法提取其生物活性化合物。使用了两种方法提取生物活性化合物：微波提取（水热转移）和浸渍提取（甲醇提取）。微波提取的结果表明，总多酚和黄酮含量以及抗氧化能力分别显著高于浸渍提取法（p < 0.05）。此外，采用喷雾干燥技术，通过壳聚糖的离子凝胶特性对提取的化合物进行包封，以增强其效果。在扫描电子显微镜（SEM）下检查了壳聚糖包封底物的物理特性，其微粒尺寸为（1.45至11.0 µm）。发现微囊化的帽蕊木、印度大麻和大麻的生物活性化合物包封效率分别为99.7%、82.3%和54.6%。因此，在壳聚糖包封叶提取物之前进行微波处理可提高生物活性化合物包封的回收率。
