Gressler V, Garbrecht M, Hossfeld D K
Blood. 1985 Dec;66(6):1482-4.
Leukemias showing a conspicuous lymphoid phenotype, ie, those that are HLA-DR positive, common acute lymphoblastic antigen (cALLA) positive, terminal deoxynucleotidyl transferase (TdT) negative, as well as myeloperoxidase positive (MPO), could be considered so-called mixed leukemias. Leukemias with biphenotypic blasts have to be distinguished from cases comprising two separate subpopulations that express different lineage-associated characteristics. By use of a simple new method (Immunogold Staining) we examined a case of chronic myelomonocytic leukemia in blastic phase and demonstrated simultaneous staining for MPO/alpha-naphthyl-esterase and expression of the HLA-DR-positive, cALLA-positive, and TdT-negative phenotype. The cALL antigen was detected only on mono- and myelo-monoblasts; its expression was inversely related to the MPO positivity, and it disappeared together with these types of blasts after chemotherapy. On the basis of our findings it remains obscure whether the cALL antigen at the initial presentation was due to the immature monocytic features of the leukemic cells or disclosed on additional lymphoid differentiation pattern of the blasts.
表现出明显淋巴样表型的白血病，即那些HLA - DR阳性、普通急性淋巴细胞抗原（cALLA）阳性、末端脱氧核苷酸转移酶（TdT）阴性以及髓过氧化物酶阳性（MPO）的白血病，可被视为所谓的混合性白血病。具有双表型原始细胞的白血病必须与包含两个表达不同谱系相关特征的独立亚群的病例相区分。通过使用一种简单的新方法（免疫金染色），我们检查了一例处于原始细胞期的慢性粒单核细胞白血病病例，并证实了MPO/α - 萘酯酶的同时染色以及HLA - DR阳性、cALLA阳性和TdT阴性表型的表达。cALL抗原仅在单核细胞和粒单核原始细胞上检测到；其表达与MPO阳性呈负相关，并且在化疗后与这些类型的原始细胞一起消失。基于我们的研究结果，最初表现时的cALL抗原是由于白血病细胞的未成熟单核细胞特征还是揭示了原始细胞额外的淋巴样分化模式仍不清楚。
