Munakata H, Isemura M, Yosizawa Z
Int J Biochem. 1985;17(10):1077-83. doi: 10.1016/0020-711x(85)90039-4.
Sulphotransferase activity in the microsomal fraction of the uterine endometrium of rabbits was measured with heparan sulphate, N-desulphated heparan sulphate and N-, O-desulphated heparan sulphate as exogenous substrates in the presence of 3'-phosphoadenylyl[35S]sulphate. Of the three acceptors, N-, O-desulphated heparan sulphate was most effectively radiolabelled. When N-, O-desulphated heparan sulphate was used as an acceptor, it was found that estrogen enhanced the sulphotransferase activity and that progesterone suppressed the effect of estrogen.
以硫酸乙酰肝素、N-去硫酸化硫酸乙酰肝素和N-、O-去硫酸化硫酸乙酰肝素作为外源性底物，在3'-磷酸腺苷[35S]硫酸盐存在的情况下，测定了兔子宫内膜微粒体部分的磺基转移酶活性。在这三种受体中，N-、O-去硫酸化硫酸乙酰肝素被最有效地放射性标记。当使用N-、O-去硫酸化硫酸乙酰肝素作为受体时，发现雌激素增强了磺基转移酶活性，而孕酮抑制了雌激素的作用。
