甘油向乳酸和乙醇酸的化学酶促转化。
Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.
作者信息
Ma Yue, Li Tianzhen, Tan Zijian, Ma Long, Liu Haifeng, Zhu Leilei
机构信息
State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science and Technology, No 9, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin, 300457, China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, National Technology Innovation Center of Synthetic Biology, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China.
出版信息
Bioresour Bioprocess. 2022 Jul 18;9(1):75. doi: 10.1186/s40643-022-00561-z.
Catalytic valorization of raw glycerol derived from biodiesel into high-value chemicals has attracted great attention. Here, we report chemoenzymatic cascade reactions that convert glycerol to lactic acid and glycolic acid. In the enzymatic step, a coenzyme recycling system was developed to convert glycerol into 1,3-dihydroxyacetone (DHA) with a yield of 92.3% in potassium phosphate buffer (300 mM, pH 7.1) containing 100 mM glycerol, 2 mM NAD, 242 U/mL glycerol dehydrogenase-GldA and NADH oxidase-SpNox at 30 °C. Subsequently, NaOH or NaClO catalyzes the formation of lactic acid and glycolic acid from DHA. The high yield of lactic acid (72.3%) and glycolic acid (78.2%) verify the benefit of the chemoenzymatic approaches.
将生物柴油衍生的粗甘油催化转化为高价值化学品引起了广泛关注。在此，我们报道了将甘油转化为乳酸和乙醇酸的化学酶级联反应。在酶促步骤中，开发了一种辅酶循环系统，在含有100 mM甘油、2 mM NAD、242 U/mL甘油脱氢酶-GldA和NADH氧化酶-SpNox的磷酸钾缓冲液（300 mM，pH 7.1）中，于30°C下将甘油转化为1,3-二羟基丙酮（DHA），产率为92.3%。随后，NaOH或NaClO催化由DHA形成乳酸和乙醇酸。乳酸（72.3%）和乙醇酸（78.2%）的高产率证实了化学酶方法的优势。
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