Borges Sandra, Piccirillo Clara, Scalera Francesca, Martins Rui, Rosa Ana, Couto José António, Almeida André, Pintado Manuela
Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005, Porto, Portugal.
Institute of Nanotechnology/NANOTEC, National Research Council, Lecce, Italy.
Bioresour Bioprocess. 2022 Mar 21;9(1):30. doi: 10.1186/s40643-022-00522-6.
The meat industry generates large amounts of by-products that are costly to be treated and discarded ecologically; moreover, they could be used to extract high added-value compounds. In this work, we present an innovative combined process which allowed the parallel extraction of both organic and mineral compounds; more specifically protein hydrolysates and single-phase hydroxyapatite were obtained. The protein hydrolysates, extracted through an enzymatic hydrolysis with alcalase, showed a degree of hydrolysis of 53.3 ± 5.1%; moreover, they had a high protein content with peptides with molecular weight lower than 1.2 kDa. Their antioxidant activities, measured with ABTS and ORAC tests, were 21.1 ± 0.5 mg ascorbic acid equivalent/g of dry extract and 87.7 ± 6.3 mg Trolox equivalent/g of dry extract, respectively. Single-phase hydroxyapatite, obtained with a simple calcination at 700 °C on the residues of the hydrolysis process, showed a Ca/P ratio close to the stoichiometric one (1.65 vs. 1.67) and presented a nanometric structure. This study reports a simple and feasible process for the valorization of porcine by-products in a large-scale up generating products with potential applications for environment remediation, biomedicine, nutrition and catalysis/bioenergy.
肉类行业产生大量副产品，这些副产品处理起来成本高昂且生态处理难度大；此外，它们还可用于提取高附加值化合物。在这项工作中，我们提出了一种创新的联合工艺，该工艺能够同时提取有机和无机化合物；更具体地说，获得了蛋白质水解物和单相羟基磷灰石。通过用碱性蛋白酶进行酶水解提取的蛋白质水解物，水解度为53.3±5.1%；此外，它们蛋白质含量高，含有分子量低于1.2 kDa的肽。用ABTS和ORAC试验测定，其抗氧化活性分别为21.1±0.5毫克抗坏血酸当量/克干提取物和87.7±6.3毫克Trolox当量/克干提取物。通过对水解过程的残渣在700℃进行简单煅烧获得的单相羟基磷灰石，其钙磷比接近化学计量比（1.65对1.67），并呈现纳米结构。本研究报道了一种简单可行的工艺，可大规模增值猪副产品，生成在环境修复、生物医学、营养和催化/生物能源方面具有潜在应用价值的产品。
