Herpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China.
Zhejiang Provincial Key Laboratory for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Sciences, Wenzhou University, Wenzhou, 325035, China.
Dev Biol. 2024 Jul;511:84-91. doi: 10.1016/j.ydbio.2024.04.005. Epub 2024 Apr 20.
We established a normal embryonic development table for the Anji salamander Hynobius amjiensis, a critically endangered tailed amphibian of the family Hynobiidae with a very limited distribution in East China, following the standards set by the early developmental table of vertebrates. Put together 32 embryonic stages for the Anji salamander was defined. The total embryonic period from oviposition to hatching is approximately 30 days at 9 °C. Stages 1-16 represent early development from cleavage to neurulation. Stages 17-32 represent organogenesis documenting later developmental events such as tail, gill, and limb formation, and hatching (Stage 32). We provided a detailed description of the external morphology and color changes of the head, trunk, limbs, tail, external gills, and balancers at various stages from egg-laying to hatching. We also described several cases of abnormal embryonic development. The establishment of the embryonic development table in H. amjiensis contributes to better understanding of the ontogeny in tailed amphibians, distinguishing closely related species, and identifying abnormal embryonic amphibians.
我们建立了安吉小鲵（Hynobius amjiensis）的正常胚胎发育表，这是一种极度濒危的有尾两栖动物，分布在中国东部，隶属于小鲵科。我们遵循了脊椎动物早期胚胎发育表的标准。共定义了 32 个安吉小鲵胚胎发育阶段。在 9°C 时，从产卵到孵化的总胚胎期约为 30 天。阶段 1-16 代表从卵裂到神经胚形成的早期发育。阶段 17-32 代表器官发生，记录了尾部、鳃和四肢形成以及孵化（阶段 32）等后期发育事件。我们详细描述了从产卵到孵化过程中头部、躯干、四肢、尾部、外部鳃和平衡器的外部形态和颜色变化。我们还描述了几种异常胚胎发育的情况。在 H. amjiensis 中建立胚胎发育表有助于更好地了解有尾两栖动物的个体发生，区分密切相关的物种，并识别异常胚胎发育的两栖动物。
