Henan University of Chinese Medicine, Zhengzhou 450046, China.
The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000.
Zhen Ci Yan Jiu. 2024 Apr 25;49(4):391-397. doi: 10.13702/j.1000-0607.20221313.
To observe the effect of electroacupuncture (EA) at "Baihui" (GV20) and "Shenting" (GV24) on the rats' behavior and the transforming precursor of brain-derived neurotrophic factor (proBDNF) into mature brain-derived neurotrophic factor (mBDNF) in the hippocampus of rats with learning and memory impairment induced by cerebral ischemia-reperfusion (IR), so as to explore its mechanisms underlying improvement of learning and memory ability.
SD rats were randomly divided into blank, sham operation, model, and EA groups, with 6 rats in each group. The model of IR was established by occlusion of the middle cerebral artery. EA (1 Hz/20 Hz) was applied to GV24 and GV20 for 30 min, once daily for 14 days. The neurological function was evaluated according to the Zea Longa's score criteria 24 h after modeling and after intervention. Morris water maze test was used to detect the learning and memory function of the rats. TTC staining was used to evaluate the cerebral infarction volume on the affected side. The protein expression levels of proBDNF, mBDNF, tissue plasminogen activator (tPA), tyrosine kinase receptor B (TrkB) and p75 neurotrophin receptor (p75NTR) in hippocampal tissue were detected by Western blot.
Compared with the sham operation group, the neurological function score, the percentage of cerebral infarction volume and the expression levels of proBDNF and p75NTR protein in hippocampus were increased (<0.01), while the times of crossing the original platform and the total distance in the target quadrant, the expression levels of mBDNF, TrkB and tPA protein and the ratio of mBDNF/proBDNF were decreased (<0.01, <0.05) in the model group. Compared with the model group, the neurological function score, the percentage of cerebral infarction volume, and the expression levels of proBDNF and p75NTR protein in hippocampus were decreased (<0.01, <0.05), while the times of crossing the original platform, the total distance in the target quadrant, and the expression levels of mBDNF, TrkB and tPA protein and the ratio of mBDNF/proBDNF were increased (<0.05, <0.01) in the EA group.
EA can alleviate learning and memory impairment in IR rats, which may be related to its function in up-regulating the expression of tPA protein and promoting the transformation of proBDNF to mBDNF, thus improving the synaptic plasticity.
观察电针（EA）“百会”（GV20）和“神庭”（GV24）对脑缺血再灌注（IR）致学习记忆障碍大鼠行为及海马区脑源性神经营养因子（proBDNF）转化为成熟脑源性神经营养因子（mBDNF）的影响，探讨其改善学习记忆能力的机制。
将 SD 大鼠随机分为空白组、假手术组、模型组和 EA 组，每组 6 只。采用大脑中动脉阻塞法建立 IR 模型。造模后 24 h 及干预后，EA 组采用 1 Hz/20 Hz 电针刺激 GV24 和 GV20，每次 30 min，每日 1 次，共 14 天。根据 ZeeLonga 评分标准评价大鼠神经功能， Morris 水迷宫检测大鼠学习记忆功能，TTC 染色法检测大鼠脑梗死体积，Western blot 法检测大鼠海马组织中 proBDNF、mBDNF、组织型纤溶酶原激活物（tPA）、酪氨酸激酶受体 B（TrkB）和 p75 神经营养因子受体（p75NTR）蛋白表达水平。
与假手术组比较，模型组大鼠神经功能评分、脑梗死体积百分比及海马组织中 proBDNF 和 p75NTR 蛋白表达水平升高（<0.01），穿越原平台次数和目标象限总距离减少（<0.01，<0.05），mBDNF、TrkB 和 tPA 蛋白表达水平及 mBDNF/proBDNF 比值降低（<0.01，<0.05）；与模型组比较，EA 组大鼠神经功能评分、脑梗死体积百分比及海马组织中 proBDNF 和 p75NTR 蛋白表达水平降低（<0.01，<0.05），穿越原平台次数、目标象限总距离增加（<0.05，<0.01），mBDNF、TrkB 和 tPA 蛋白表达水平及 mBDNF/proBDNF 比值升高（<0.05，<0.01）。
EA 可改善 IR 大鼠学习记忆障碍，其作用机制可能与上调 tPA 蛋白表达，促进 proBDNF 向 mBDNF 转化，从而改善突触可塑性有关。
