Holmes P V, Sogn J, Schillinger E, Janson P O
J Reprod Fertil. 1985 Nov;75(2):393-9. doi: 10.1530/jrf.0.0750393.
Rabbit ovaries were isolated surgically before the ovulatory gonadotrophin stimulation and perfused in vitro. Untreated, control ovaries never ovulated. Ovaries treated in vitro with ovine LH ovulated 10-14 h later and the oocytes had undergone germinal vesicle breakdown (GVB). LH induced increases in progesterone secretion from the treated ovaries. A 3 beta-hydroxysteroid dehydrogenase blocker ('Compound A') effectively reduced progesterone secretion into the perfusate and follicular fluid to very low levels but had no effect on ovulation rate or on oocyte maturation. Excessively high progesterone levels were obtained artificially in perfusates by addition of exogenous steroid; the number of ovaries ovulating was markedly reduced but there was no effect on oocyte maturation. It is concluded that the rise in progesterone that normally occurs immediately after the LH surge is not a prerequisite for ovulation in the rabbit. However, progesterone may have a modifying effect on LH-induced follicle rupture when at a pharmacologically high level.
在排卵性促性腺激素刺激前，通过手术分离兔卵巢并进行体外灌注。未经处理的对照卵巢从不排卵。体外经羊促黄体生成素（LH）处理的卵巢在10 - 14小时后排卵，卵母细胞发生了生发泡破裂（GVB）。LH诱导处理后的卵巢孕酮分泌增加。一种3β - 羟基类固醇脱氢酶阻滞剂（“化合物A”）有效地将孕酮分泌到灌注液和卵泡液中的水平降低到非常低的水平，但对排卵率或卵母细胞成熟没有影响。通过添加外源性类固醇在灌注液中人为获得过高的孕酮水平；排卵的卵巢数量明显减少，但对卵母细胞成熟没有影响。得出的结论是，在兔中，LH峰后立即正常发生的孕酮升高不是排卵的先决条件。然而，当孕酮处于药理学高水平时，可能对LH诱导的卵泡破裂有调节作用。
