Lin F K, Suggs S, Lin C H, Browne J K, Smalling R, Egrie J C, Chen K K, Fox G M, Martin F, Stabinsky Z
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7580-4. doi: 10.1073/pnas.82.22.7580.
The human erythropoietin gene has been isolated from a genomic phage library by using mixed 20-mer and 17-mer oligonucleotide probes. The entire coding region of the gene is contained in a 5.4-kilobase HindIII-BamHI fragment. The gene contains four intervening sequences (1562 base pairs) and five exons (582 base pairs). It encodes a 27-amino acid signal peptide and a 166-amino acid mature protein with a calculated Mr of 18,399. The erythropoietin gene, when introduced into Chinese hamster ovary cells, produces erythropoietin that is biologically active in vitro and in vivo.
通过使用混合的20聚体和17聚体寡核苷酸探针,从基因组噬菌体文库中分离出了人类促红细胞生成素基因。该基因的整个编码区包含在一个5.4千碱基的HindIII - BamHI片段中。该基因含有四个间隔序列(1562个碱基对)和五个外显子(582个碱基对)。它编码一个27个氨基酸的信号肽和一个166个氨基酸的成熟蛋白,计算所得的分子量为18399。当将促红细胞生成素基因导入中国仓鼠卵巢细胞时,所产生的促红细胞生成素在体外和体内均具有生物活性。