Lin F K, Lin C H, Lai P H, Browne J K, Egrie J C, Smalling R, Fox G M, Chen K K, Castro M, Suggs S
Gene. 1986;44(2-3):201-9. doi: 10.1016/0378-1119(86)90183-6.
The erythropoietin (Epo) gene from Cynomolgus monkeys has been isolated from a kidney cDNA library using mixed 20-mer oligodeoxynucleotide probes. The gene encodes a 168 amino acid (aa) mature protein with a calculated Mr of 18,490 and a presumptive signal peptide of 24 aa. The Epo gene, when transfected into Chinese hamster ovary (CHO) cells, produces a glycosylated protein with an apparent Mr of 34,000. The expressed product is biologically active in vivo. The monkey gene exhibits 92% and 94% homology to the human gene at the aa and nucleotide sequence levels, respectively. When compared with the human Epo, monkey Epo has an additional 3-aa residue at the N terminus of the mature protein and a deletion of an internal lysine residue.
已使用混合的20聚体寡脱氧核苷酸探针从食蟹猴的肾脏cDNA文库中分离出促红细胞生成素（Epo）基因。该基因编码一种168个氨基酸（aa）的成熟蛋白，计算所得的分子量为18,490，还有一个推测的24个氨基酸的信号肽。将Epo基因转染到中国仓鼠卵巢（CHO）细胞中时，会产生一种表观分子量为34,000的糖基化蛋白。表达产物在体内具有生物活性。猴基因在氨基酸和核苷酸序列水平上与人基因的同源性分别为92%和94%。与人类Epo相比，猴Epo在成熟蛋白的N端有一个额外的3个氨基酸残基，并且缺失一个内部赖氨酸残基。
