ARC Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.
Plant J. 2024 Jul;119(1):445-459. doi: 10.1111/tpj.16775. Epub 2024 Apr 23.
The lycophyte Phylloglossum drummondii is the sole inhabitant of its genus in the Huperzioideae group and one of a small minority of plants which perform uridine to cytidine RNA editing. We assembled the P. drummondii chloroplast and mitochondrial genomes and used RNA sequence data to build a comprehensive profile of organellar RNA editing events. In addition to many C-to-U editing events in both organelles, we found just four U-to-C editing events in the mitochondrial transcripts cob, nad1, nad5 and rpl2. These events are conserved in related lycophytes in the genera Huperzia and Phlegmariurus. De novo transcriptomes for three of these lycophytes were assembled to search for putative U-to-C RNA editing enzymes. Four putative U-to-C editing factors could be matched to the four mitochondrial U-to-C editing sites. Due to the unusually few numbers of U-to-C RNA editing sites, P. drummondii and related lycophytes are useful models for studying this poorly understood mechanism.
石松目木贼科 Phylloglossum 属的单种植物鳞叶卷柏是该属在木贼亚属中的唯一代表，也是少数能够进行尿嘧啶到胞嘧啶 RNA 编辑的植物之一。我们组装了鳞叶卷柏的叶绿体和线粒体基因组，并利用 RNA 序列数据构建了一个全面的细胞器 RNA 编辑事件图谱。除了两个细胞器中许多 C 到 U 的编辑事件外，我们还在线粒体转录物 cob、nad1、nad5 和 rpl2 中发现了仅四个 U 到 C 的编辑事件。这些事件在相关的石松目属 Huperzia 和 Phlegmariurus 中是保守的。为了搜索潜在的 U 到 C RNA 编辑酶，我们为其中的三种石松目植物组装了从头转录组。可以将四个假定的 U 到 C 编辑因子与四个线粒体 U 到 C 编辑位点相匹配。由于 U 到 C RNA 编辑位点的数量异常少，鳞叶卷柏和相关的石松目植物是研究这种了解甚少的机制的有用模型。
