Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, Hokkaido 050-8585, Japan; Department of Sciences and Informatics, Course of Chemical and Biological Systems, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan.
Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
J Hazard Mater. 2024 Jun 5;471:134348. doi: 10.1016/j.jhazmat.2024.134348. Epub 2024 Apr 18.
This study ventures into the exploration of potential poly-3-hydroxybutyrate (PHB) degradation in alpine environments. PHB-degrading bacteria were identified in both campus soil, representing a residential area, and Mt. Kurodake soil, an alpine region in Hokkaido, Japan. Next-generation sequencing analysis indicated that the campus soil exhibited higher microbial diversity, while Ralstonia insidiosa C1, isolated from Mt. Kurodake soil, displayed the highest proficiency in PHB degradation. R. insidiosa C1 efficiently degraded up to 3% (w/v) of PHB and various films composed of other biopolymers at 14 °C. This bacterium synthesized homopolymers using substrates such as 3-hydroxybutyric acid, sugars, and acetic acid, while also produced copolymers using a mixture of fatty acids. The analysis results confirmed that the biopolymer synthesized by strain C1 using glucose was PHB, with physical properties comparable to commercial products. The unique capabilities of R. insidiosa C1, encompassing both the production and degradation of bioplastics, highlight its potential to establish a novel material circulation model.
本研究探索了聚 3-羟基丁酸酯（PHB）在高山环境中的潜在降解情况。在校园土壤（代表居民区）和日本北海道黑岳山的高山土壤中均鉴定到了 PHB 降解细菌。下一代测序分析表明，校园土壤表现出更高的微生物多样性，而从黑岳山土壤中分离出的 Ralstonia insidiosa C1 则显示出最高的 PHB 降解能力。R. insidiosa C1 能够在 14°C 下高效降解高达 3%（w/v）的 PHB 以及其他各种生物聚合物薄膜。该细菌使用 3-羟基丁酸、糖和乙酸等底物合成均聚物，同时使用脂肪酸混合物生产共聚物。分析结果证实，菌株 C1 使用葡萄糖合成的生物聚合物为 PHB，其物理性质可与商业产品媲美。R. insidiosa C1 兼具生物塑料的生产和降解能力，这突显了其建立新型材料循环模型的潜力。
