单糖分析一锅衍生化后反相液相色谱分离和紫外/可见检测。
Monosaccharide Analysis After One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection.
机构信息
Plant Functional Genomics Lab, Institute of Molecular Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
Department of Pharmaceutical Sciences, Division of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria.
出版信息
Methods Mol Biol. 2024;2788:67-79. doi: 10.1007/978-1-0716-3782-1_5.
Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensitivity (limit of quantitation 1 nmol). This method allows for complex monosaccharide mixtures to be separated and quantified using a reasonably simple and safe derivatization procedure.
用 1-苯基-3-甲基-5-吡唑啉酮（PMP）对单糖进行衍生化，每个糖分子引入两个生色团。使用普通的液相色谱设备，在表面多孔的 C18 反相柱上进行分离，可实现短的分析时间（<20 分钟）和高灵敏度（定量限为 1 毫摩尔）。该方法允许使用相当简单和安全的衍生化程序分离和定量复杂的单糖混合物。
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