Zhang Lingyi, Xu Jin, Zhang Lihua, Zhang Weibing, Zhang Yukui
National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116011, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Aug 5;793(1):159-65. doi: 10.1016/s1570-0232(03)00373-8.
In this paper, the method for the derivatization of carbohydrates with 1-phenyl-3-methyl-5-pyrazolone (PMP) was simplified. One-third of the derivatization time was saved. Five monosaccharide derivatives have been well separated by MEKC and HPLC under optimized conditions. Good reproducibility could be obtained with relative standard deviation (RSD) values of the migration times within 5.0 and 2.3%, respectively. Furthermore, the developed methods have been successfully applied to the analysis of carbohydrates in Aloe powder and food. These methods are quite useful for routine analysis of monosaccharides and oligosaccharides in real samples.
本文简化了用1-苯基-3-甲基-5-吡唑啉酮（PMP）对碳水化合物进行衍生化的方法。节省了三分之一的衍生化时间。在优化条件下，5种单糖衍生物已通过毛细管电泳（MEKC）和高效液相色谱（HPLC）得到很好的分离。迁移时间的相对标准偏差（RSD）值分别在5.0%和2.3%以内，可获得良好的重现性。此外，所建立的方法已成功应用于芦荟粉和食品中碳水化合物的分析。这些方法对于实际样品中单糖和寡糖的常规分析非常有用。
