Fernández Jimena, Bruno-Galarraga María Macarena, Cueto Marcela Isabel, Bonadeo Nadia, Notaro Ulises, Soto Andrés Telésforo, de la Sota Rodolfo Luzbel, Salvetti Natalia Raquel, Bianchi Carolina Paula, Cristina Carolina, Ortega Hugo Héctor, Gibbons Alejandro Eduardo, Lacau-Mengido Isabel María
Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Facultad de Ciencias Veterinarias, PROANVET, Tandil, Buenos Aires, Argentina; CIVETAN, UNCPBA-CICPBA-CONICET, Tandil, Buenos Aires, Argentina.
Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche, Laboratorio de Reproducción de Rumiantes Menores. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (INTA-CONICET), Bariloche, Argentina.
Anim Reprod Sci. 2024 Jun;265:107474. doi: 10.1016/j.anireprosci.2024.107474. Epub 2024 Apr 15.
This study investigated the effect of hCG or GnRH on structural changes of the corpora lutea (CL) and the regulation of the expression of steroidogenic enzymes involved in P secretion in post-ovulatory (po-CL) and accessory CL (acc-CL). Sixty-four ewes were assigned to three groups receiving: 300 IU of hCG (hCG) or 4 µg Buserelin (GnRH) or 1 mL of saline solution (Control) on Day (d) 4 post artificial insemination (FTAI). Laparoscopic ovarian were performed on d 4, 14 and, 21 post-FTAI to determine the numbers of CL. Blood samples were collected for serum LH and P analysis. On d 14 post-FTAI, both CL were removed from the ovary to determine large luteal cell (LLC) number and to evaluate the expression of steroidogenic enzymes (HSD3B1, STAR, CYP11A1). Only hCG and GnRH treated ewes generated acc-CL. The LLC in both po- and acc-CL were significantly greater in the hCG group compared to GnRH and Control groups (P<0.05). Overall, hCG group showed the greatest immunodetection of HSD3B1and STAR in both po- and acc-CL (P<0.05). rnRNA expression of HSD3B1, STAR and CYP11A1 in the acc-CL tended to be greater in hCG group than in GnRH group (P<0.1). The LH concentration was increased in GnRH group (P<0.05) and P concentration was greater in hCG group compared to the other groups (P<0.05). In conclusion, administration of hCG has a notably impact on acc-CL development and the expression of steroidogenic enzymes compared to GnRH treatment in ewes. This leads to elevated P concentration and improved luteal function.
本研究调查了人绒毛膜促性腺激素（hCG）或促性腺激素释放激素（GnRH）对排卵后黄体（po-CL）和副黄体（acc-CL）中黄体（CL）结构变化的影响以及参与孕酮（P）分泌的类固醇生成酶表达的调控。64只母羊被分为三组，在人工授精（FTAI）后第4天分别接受：300 IU的hCG（hCG组）或4 µg布舍瑞林（GnRH组）或1 mL盐溶液（对照组）。在FTAI后第4、14和21天进行腹腔镜卵巢检查以确定CL的数量。采集血样进行血清促黄体生成素（LH）和P分析。在FTAI后第14天，从卵巢中取出两个CL以确定大黄体细胞（LLC）数量并评估类固醇生成酶（3β-羟基类固醇脱氢酶1型，HSD3B1；类固醇生成急性调节蛋白，STAR；细胞色素P450侧链裂解酶，CYP11A1）的表达。只有hCG和GnRH处理的母羊产生了acc-CL。与GnRH组和对照组相比，hCG组po-CL和acc-CL中的LLC数量均显著更多（P<0.05）。总体而言，hCG组在po-CL和acc-CL中HSD3B1和STAR的免疫检测结果最强（P<0.05）。hCG组acc-CL中HSD3B1、STAR和CYP11A1的mRNA表达倾向于高于GnRH组（P<0.1）。GnRH组的LH浓度升高（P<0.05），与其他组相比，hCG组的P浓度更高（P<0.05）。总之，与GnRH处理相比，hCG给药对母羊acc-CL发育和类固醇生成酶表达有显著影响。这导致P浓度升高和黄体功能改善。
