Fisheries Science Institute, Chonnam National University, Yeosu 59626, South Korea.
Fisheries Science Institute, Chonnam National University, Yeosu 59626, South Korea; Department of Ocean Integrated Science, Chonnam National University, Yeosu 59626, South Korea.
Environ Toxicol Pharmacol. 2024 Jun;108:104456. doi: 10.1016/j.etap.2024.104456. Epub 2024 Apr 22.
Di(2-ethylhexyl) phthalate (DEHP), have been increasingly used as plasticizers to manufacture soft and flexible materials and ubiquitously found in water and sediments in the aquatic ecosystem. The aim of the present study was to evaluate the effect of DEHP exposure on cellular homeostasis (HSF1 and seven HSPs), immune responses (ILF), and apoptotic responses (p53, BAX, Bcl-2). DEHP exposure upregulated the expression of HSF1 and ILF. Moreover, it altered the expression levels of HSPs (upregulation of HSP70, HSP90, HSP40, HSP83, and HSP67B2 and downregulation of HSP60 and HSP21) in conjunction with HSF1 and ILF in the gills and hepatopancreas of M. japonicus exposed to DEHP. At the protein level, DEHP exposure changed apoptotic signals in both tissues of M. japonicus. These findings indicate that chronic exposures to several DEHP concentrations could disturb cellular balance, damage the inflammatory and immune systems, and induce apoptotic cell death, thereby affecting the survival of M. japonicus.
邻苯二甲酸二（2-乙基己基）酯（DEHP）作为增塑剂，广泛用于制造柔软、有弹性的材料，在水生生态系统的水和沉积物中无处不在。本研究旨在评估 DEHP 暴露对细胞内稳态（HSF1 和七种 HSPs）、免疫反应（ILF）和细胞凋亡反应（p53、BAX、Bcl-2）的影响。DEHP 暴露上调了 HSF1 和 ILF 的表达。此外，它改变了 HSPs 的表达水平（HSP70、HSP90、HSP40、HSP83 和 HSP67B2 的上调和 HSP60 和 HSP21 的下调）与 DEHP 暴露的日本沼虾鳃和肝胰腺中的 HSF1 和 ILF 一起。在蛋白质水平上，DEHP 暴露改变了日本沼虾两种组织中的凋亡信号。这些发现表明，慢性暴露于几种 DEHP 浓度可能会破坏细胞平衡，损害炎症和免疫系统，并诱导细胞凋亡死亡，从而影响日本沼虾的生存。
