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[空间转录组学技术及其在植物研究中的应用]

[Spatial transcriptomics techniques and its applications in plant research].

作者信息

Zhang Cheng, Zhou Chengzhe, Tian Caiyun, Guo Yuqiong

机构信息

College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.

Anxi College of Tea Science (College of Digital Economy), Fujian Agriculture and Forestry University, Quanzhou 362400, Fujian, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2024 Apr 25;40(4):971-987. doi: 10.13345/j.cjb.230662.

Abstract

The heterogeneity of gene expression in plant cells plays a crucial role in determining the functional differences among tissues. Recent advancements in spatial transcriptome (ST) technology have significantly contributed to the study of specific biological questions in plants. This technology has been successfully applied to examine cell development, identification, and stress resistance. This review aims to explore the application of ST technology in plants by reviewing three aspects: the development of ST technology, its current application in plants, and future research directions. The review provides a systematic description of the development process of ST technology, with a focus on analyzing its progress in studying plant cell growth and differentiation, plant cell identification, and stress resistance. In addition, the challenges faced by ST technology in plant applications are summarized, along with proposed future directions for plant research, including the advantages of combining other omics technologies with ST technology to tackle scientific challenges in the field of plants.

摘要

植物细胞中基因表达的异质性在决定组织间功能差异方面起着关键作用。空间转录组(ST)技术的最新进展对植物特定生物学问题的研究做出了重大贡献。该技术已成功应用于细胞发育、鉴定和抗逆性研究。本综述旨在通过回顾三个方面来探讨ST技术在植物中的应用:ST技术的发展、其在植物中的当前应用以及未来研究方向。该综述系统地描述了ST技术的发展过程,重点分析了其在研究植物细胞生长和分化、植物细胞鉴定以及抗逆性方面的进展。此外,总结了ST技术在植物应用中面临的挑战,以及提出的植物研究未来方向,包括将其他组学技术与ST技术相结合以应对植物领域科学挑战的优势。

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