Fujii Y
Hokkaido Igaku Zasshi. 1985 Sep;60(5):735-47.
The aim is to examine experimentally whether injections of LPS in combination with Daunomycin are effective on a rat myelomonocytic leukemia. Effects of Lipopolysaccharide (LPS), one of the major differentiation-inducing agents in the mouse myeloid leukemia cell line M1, were investigated on the cells of a rat myelomonocytic leukemia cell line c-WRT-7 in vitro and in vivo. It was shown that sensitive cells of c-WRT-7 cells changed remarkably into macrophage-like cells, which lost the growth potential by LPS-treatment, whereas such change and growth inhibition in insensitive cells of c-WRT-7 were not so remarkable by LPS-treatment. Although the sensitive cells were extremely malignant in vivo, the sensitive cells treated with LPS in vitro lost the transplantability into the syngeneic rats. The sensitive cells by injections of LPS developed morphologically into macrophage-like cells in diffusion chamber i.p. in syngeneic rat. Injections of LPS inhibited the progression of leukemia in 60% of the rats which had been inoculated with the sensitive cells, whereas the leukemia-development was inhibited in only 20% of the rats which had been inoculated with the insensitive cells and i.p. treated with LPS. Daunomycin, Aclacinomycin A and Vincristine induced phagocytic activities in c-WRT-7 cells, whereas Actinomycin D and Cycloheximide showed no such effects. Daunomycin in combination with LPS increased the number of phagocytic cells, whereas cycloheximide inhibited the LPS induced phagocytosis. Injections of LPS in combination with Daunomycin inhibited doubtlessly the leukemia-development in rats which had been i.p. inoculated with the sensitive cells, compared to the injections of LPS or Daunomycin alone. In conclusion, it is suggested that the effects of LPS and Daunomycin on the inhibition of the leukemia-development could be associated in part with their differentiation-inducing activities.
目的是通过实验研究脂多糖（LPS）联合柔红霉素对大鼠骨髓单核细胞白血病是否有效。研究了脂多糖（LPS），即小鼠髓系白血病细胞系M1中主要的分化诱导剂之一，对大鼠骨髓单核细胞白血病细胞系c-WRT-7细胞的体内外作用。结果表明，c-WRT-7细胞的敏感细胞经LPS处理后显著转变为巨噬细胞样细胞，失去了生长潜能，而c-WRT-7不敏感细胞经LPS处理后的这种变化和生长抑制并不明显。虽然敏感细胞在体内具有极高的恶性，但体外经LPS处理的敏感细胞失去了对同基因大鼠的移植能力。经LPS注射的敏感细胞在同基因大鼠腹腔内的扩散室中形态上发展为巨噬细胞样细胞。LPS注射抑制了60%接种敏感细胞的大鼠白血病进展，而接种不敏感细胞并经腹腔注射LPS处理的大鼠中，只有20%的白血病发展受到抑制。柔红霉素、阿克拉霉素A和长春新碱可诱导c-WRT-7细胞的吞噬活性，而放线菌素D和环己酰亚胺则无此作用。柔红霉素与LPS联合使用可增加吞噬细胞数量，而环己酰亚胺则抑制LPS诱导的吞噬作用。与单独注射LPS或柔红霉素相比，LPS联合柔红霉素注射无疑抑制了腹腔接种敏感细胞的大鼠的白血病发展。总之，提示LPS和柔红霉素对白血病发展的抑制作用可能部分与其分化诱导活性有关。
