Mowahedi Masouma, Aramesh Azam, Khouzani Mozhgan Sorkhi, Khouzani Marjan Sorkhi, Daryanoush Saeed, Samet Mohammad, Samadi Morteza
Abortion Research Center, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Thalassemia and Hemophilia Research Center, Shahid Dastgheib Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Open Respir Med J. 2024 Jan 10;18:e18743064266613. doi: 10.2174/0118743064266613231123103523. eCollection 2024.
Asthma is one of the respiratory disorders caused by chronic airway inflammation. IL-4 has been identified as one of the participating interleukins in the severity of asthma.
A case-control study was conducted to determine the association of rs1805010, a single nucleotide polymorphism in the interleukin 4 receptor α chain, with asthma and immunoglobulin E and IL-17A serum levels in Iranian populations.
ELISA was used to investigate the relationship between three different varieties of SNP I50V and serum IL-17A levels, as well as total IgE levels. Based on GINA criteria, patients were classified into mild, moderate, and severe groups based on the association between SNP I50V, IL-17A, and total IgE. In order to analyze the data, the student-t-test and the one-way ANOVA were used.
The SNP I50V was associated with asthma in a significant way (p = 0.001). IL-17A and total IgE levels were significantly higher in asthmatic patients than in control participants (p 0.05 and p 0.021, respectively), but neither showed any association with SNP I50V in the asthmatic patients.
Asthma patients have a higher prevalence of the I allele, reflecting the significance of Th2 cells. Although total IgE and IL-17A levels increased in both disease subgroups, total IgE level augmentation correlates directly with disease severity, while IL-17A level enhancement does not.
哮喘是一种由慢性气道炎症引起的呼吸系统疾病。白细胞介素-4（IL-4）已被确定为参与哮喘严重程度的白细胞介素之一。
进行一项病例对照研究，以确定白细胞介素4受体α链单核苷酸多态性rs1805010与伊朗人群哮喘、免疫球蛋白E及IL-17A血清水平之间的关联。
采用酶联免疫吸附测定法（ELISA）研究三种不同类型的单核苷酸多态性I50V与血清IL-17A水平以及总免疫球蛋白E水平之间的关系。根据全球哮喘防治创议（GINA）标准，基于单核苷酸多态性I50V、IL-17A和总免疫球蛋白E之间的关联，将患者分为轻度、中度和重度组。为分析数据，使用了学生t检验和单因素方差分析。
单核苷酸多态性I50V与哮喘显著相关（p = 0.001）。哮喘患者的IL-17A和总免疫球蛋白E水平显著高于对照组参与者（分别为p < 0.05和p < 0.021），但在哮喘患者中两者均与单核苷酸多态性I50V无任何关联。
哮喘患者中I等位基因的患病率较高，这反映了Th2细胞的重要性。虽然在两个疾病亚组中总免疫球蛋白E和IL-17A水平均升高，但总免疫球蛋白E水平的升高与疾病严重程度直接相关，而IL-17A水平的升高则不然。
