Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; Medical Biochemistry and Molecular Biology Department, Armed Forces College of Medicine (AFCM), Cairo, Egypt.
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Cytokine. 2022 Sep;157:155933. doi: 10.1016/j.cyto.2022.155933. Epub 2022 Jun 18.
Asthma is chronic immune-mediated airway inflammation, and it is affected by a complex network of interacting cytokines. To date, the exact role of each cytokine and its genetic polymorphisms in childhood asthma development and its severity has remained poorly understood. The purpose of this study was to explore potential roles of four cytokine genes polymorphism and serum levels l [(T helper-2 (Th2) cytokine); Interleukin-4 (IL-4) 590, (Th3 cytokine); and transforming growth factor β1 (TGF-β1) 509T; (Th17) including tumor necrosis factor-alpha (TNF-α), and IL17A rs8193036] in childhood asthma risk and control in Egyptian children, for the 1st time.
This case-control study included two children subgroups; Group1 included 216 non-asthmatic controls and (Group 2) 216 cases diagnosed with asthma (clinically and spirometry-based) were classified as controlled, partly controlled, and uncontrolled. Polymorphisms of TGF-β1-509, IL-4 590, and TNF-α-308 genes were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). IL-17 was genotyped using tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR). Serum cytokines levels were measured by enzyme-linked immunosorbent assay (ELISA).
Serum total IgE, TGF-β1, IL4, TNF-α, and IL17A levels were significantly higher in asthmatic compared to controls. Also, significant increases in serum total IgE, IL-4, TGF-β1, and TNF-α levels are combined with poor asthma control, while no significant IL17A changes. There were significant changes of IL-4-590, TNF-α-308, and IL17A genotypes and allele distributions between asthmatic and controls groups as well as different asthma control levels; while no impact of TGF-β1 SNP on asthma risk and control level. Four cytokines SNPs affected their serum levels among asthmatic patients.
There are impacts of cytokine gene polymorphisms (IL-4-590, TNF-α-308, and IL17A); but not TGF-β1 on asthma susceptibility and poor asthma control in Egyptian children.
哮喘是一种慢性免疫介导的气道炎症，受相互作用的细胞因子复杂网络的影响。迄今为止，每种细胞因子及其遗传多态性在儿童哮喘发病机制及其严重程度中的确切作用仍知之甚少。本研究旨在首次探讨四种细胞因子基因多态性及其血清水平[(辅助性 T 细胞-2 (Th2)细胞因子);白细胞介素-4 (IL-4)590、(Th3 细胞因子);和转化生长因子-β1 (TGF-β1)509T;(Th17)包括肿瘤坏死因子-α(TNF-α)和 IL17A rs8193036]在埃及儿童哮喘发病风险和控制中的潜在作用。
这项病例对照研究包括两个儿童亚组;第 1 组包括 216 名非哮喘对照和(第 2 组)216 名经临床和肺活量测定诊断为哮喘的病例，分为控制组、部分控制组和未控制组。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测 TGF-β1-509、IL-4 590 和 TNF-α-308 基因的多态性。采用四引物扩增阻滞突变系统聚合酶链反应(ARMS-PCR)对 IL-17 进行基因分型。采用酶联免疫吸附试验(ELISA)检测血清细胞因子水平。
与对照组相比，哮喘患者的血清总 IgE、TGF-β1、IL4、TNF-α和 IL17A 水平显著升高。此外，血清总 IgE、IL-4、TGF-β1 和 TNF-α水平的显著升高与哮喘控制不良有关，而 IL17A 水平无显著变化。哮喘组与对照组以及不同哮喘控制水平之间，IL-4-590、TNF-α-308 和 IL17A 基因型和等位基因分布均有显著变化;而 TGF-β1 SNP 对哮喘发病风险和控制水平无影响。四种细胞因子 SNPs 影响哮喘患者的血清水平。
细胞因子基因多态性(IL-4-590、TNF-α-308 和 IL17A)而非 TGF-β1 影响埃及儿童哮喘易感性和哮喘控制不良。
