Kostiala A A, Jormalainen S, Kosunen T U
Am J Clin Pathol. 1979 Sep;72(3):437-43. doi: 10.1093/ajcp/72.3.437.
Rabbits received intravenous injections of bacteria or fungi, and a comparison was made of the abilities of broth cultures, plating after dilution either in saline solution or in distilled water containing Triton X-100, and buffy coat examinations to detect the organisms in heart blood. The most sensitive method was broth culture. By microscopy or subculture of buffy coat cells prepared by centrifugation of blood in microhematocrit tubes, organisms were rapidly and regularly detected when their viable counts increased to 300--1,000/ml as detected by plating. By micromodification, buffy coat examination is technically easy to perform, and the method is only slightly less sensitive than when a larger amount of blood is used. Thus, it would be ideal for rapid provisional diagnosis of sepsis in patients, e.g., neonates, when the use of only a small blood sample is preferred.
给兔子静脉注射细菌或真菌，比较肉汤培养、在含吐温X - 100的生理盐水或蒸馏水中稀释后平板接种以及血沉棕黄层检查检测心血中微生物的能力。最敏感的方法是肉汤培养。通过显微镜检查或对微量血细胞比容管中血液离心制备的血沉棕黄层细胞进行传代培养，当通过平板接种检测到活菌落数增加到300 - 1000/ml时，能快速且规律地检测到微生物。通过微改良，血沉棕黄层检查在技术上易于操作，该方法的敏感性仅略低于使用大量血液时的敏感性。因此，对于仅需采集少量血样的患者（如新生儿）败血症的快速初步诊断而言，它将是理想的方法。
