State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, PR China; School of Physical Education and Health Management, Henan Finance University, Zhengzhou, 450046, Henan, PR China.
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, Henan, PR China.
Biochem Biophys Res Commun. 2024 Jun 25;714:149956. doi: 10.1016/j.bbrc.2024.149956. Epub 2024 Apr 18.
Maize is a major cereal crop world widely, however, the yield of maize is frequently limited by dehydration and even death of plants, which resulted from osmotic stress such as drought and salinity. Dissection of molecular mechanisms controlling stress tolerance will enable plant scientists and breeders to increase crops yield by manipulating key regulatory components.
The candidate OSR1 gene was identified by map-based cloning. The expression level of OSR1 was verified by qRT-PCR and digital PCR in WT and osr1 mutant. Electrophoretic mobility shift assay, transactivation activity assay, subcellular localization, transcriptome analysis and physiological characters measurements were conducted to analyze the function of OSR1 in osmotic stress resistance in maize.
The osr1 mutant was significantly less sensitive to osmotic stress than the WT plants and displayed stronger water-holding capacity, and the OSR1 homologous mutant in Arabidopsis showed a phenotype similar with maize osr1 mutant. Differentially expressed genes (DEGs) were identified between WT and osr1 under osmotic stress by transcriptome analysis, the expression levels of many genes, such as LEA, auxin-related factors, PPR family members, and TPR family members, changed notably, which may primarily involve in osmotic stress or promote root development.
OSR1 may serve as a negative regulatory factor in response to osmotic stress in maize. The present study sheds new light on the molecular mechanisms of osmotic stress in maize.
玉米是世界范围内一种主要的谷类作物，但玉米的产量经常受到干旱和盐渍等渗透胁迫的限制，导致植物脱水甚至死亡。解析控制胁迫耐受的分子机制将使植物科学家和培育者能够通过操纵关键调控元件来提高作物产量。
通过图谱克隆鉴定候选 OSR1 基因。通过 qRT-PCR 和数字 PCR 在 WT 和 osr1 突变体中验证 OSR1 的表达水平。进行电泳迁移率变动分析、转录激活活性分析、亚细胞定位、转录组分析和生理特性测量，以分析 OSR1 在玉米渗透胁迫抗性中的功能。
osr1 突变体对渗透胁迫的敏感性明显低于 WT 植物，表现出更强的持水能力，拟南芥中的 OSR1 同源突变体表现出与玉米 osr1 突变体相似的表型。通过转录组分析鉴定出 WT 和 osr1 在渗透胁迫下的差异表达基因（DEGs），许多基因如 LEA、生长素相关因子、PPR 家族成员和 TPR 家族成员的表达水平发生显著变化，这些基因可能主要参与渗透胁迫或促进根系发育。
OSR1 可能作为玉米响应渗透胁迫的负调控因子。本研究为玉米渗透胁迫的分子机制提供了新的视角。
