生长育肥猪糙米中氨基酸消化率的测定与预测

Determination and prediction of amino acid digestibility in brown rice for growing-finishing pigs.

作者信息

Ouyang Qing, Li Rui, Feng Ganyi, Hou Gaifeng, Jiang Xianji, Liu Xiaojie, Tang Hui, Long Ciming, Yin Jie, Yin Yulong

机构信息

College of Animal Science and Technology, Hunan Agricultural University, Hunan Co- Innovation Center of Animal Production Safety, Changsha 410128, China.

Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Poultry Breeding Pollution Control and Resource Technology, Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.

出版信息

Anim Biosci. 2024 Aug;37(8):1474-1482. doi: 10.5713/ab.23.0455. Epub 2024 Apr 24.

DOI:10.5713/ab.23.0455

PMID:38665090

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11222864/

Abstract

OBJECTIVE

The experiment aimed to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 brown rice samples fed to pigs, and to construct predictive models for SID of CP and AA based on the physical characteristics and chemical composition of brown rice.

METHODS

Twenty-two cannulated pigs (initial body weight: 42.0±1.2 kg) were assigned to a replicated 11×3 incomplete Latin square design, including an N-free diet and 10 brown rice diets. Each period included 5 d adaptation and 2 d ileal digesta collection. Chromic oxide was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility.

RESULTS

The coefficients of variation of all detected indices for physical characteristics and chemical composition, except for bulk weight, dry matter (DM) and gross energy, in 10 brown rice samples were greater than 10%. The SID of CP, lysine (Lys), methionine, threonine (Thr), and tryptophan (Trp) in brown rice was 77.2% (62.6% to 85.5%), 87.5% (80.3% to 94.3%), 89.2% (78.9% to 98.9%), 55.4% (46.1% to 67.6%) and 92.5% (86.3% to 96.3%), respectively. The best prediction equations for the SID of CP, Lys, Thr, and Trp were as following, SIDCP = -664.181+8.484×DM (R2 = 0.40), SIDLys = 53.126+6.031×ether extract (EE)+0.893×thousand-kernel volume (R2 = 0.66), SIDThr = 39.916+7.843×EE (R2 = 0.41), and SIDTrp = -361.588+4.891×DM+0.387×total starch (R2 = 0.85).

CONCLUSION

Overall, a great variation exists among 10 sources of brown rice, and the thousand-grain volume, DM, EE, and total starch can be used as the key predictors for SID of CP and AA.

摘要

目的

本试验旨在测定10种饲喂猪的糙米样品中粗蛋白（CP）和氨基酸（AA）的标准回肠消化率（SID），并基于糙米的物理特性和化学成分构建CP和AA的SID预测模型。

方法

22头安装了瘘管的猪（初始体重：42.0±1.2 kg）被分配到一个重复的11×3不完全拉丁方设计中，包括无氮日粮和10种糙米日粮。每个阶段包括5天的适应期和2天的回肠食糜收集期。在所有日粮中添加0.3%的氧化铬作为不可消化标记物，用于计算回肠CP和AA消化率。

结果

10种糙米样品中，除容重、干物质（DM）和总能外，所有检测的物理特性和化学成分指标的变异系数均大于10%。糙米中CP、赖氨酸（Lys）、蛋氨酸、苏氨酸（Thr）和色氨酸（Trp）的SID分别为77.2%（62.6%至85.5%）、87.5%（80.3%至94.3%）、89.2%（78.9%至98.9%）、55.4%（46.1%至67.6%）和92.5%（86.3%至96.3%）。CP, Lys, Thr和Trp的SID的最佳预测方程如下：SIDCP = -664.181+8.484×DM (R2 = 0.40)，SIDLys = 53.126+6.031×乙醚提取物（EE）+0.893×千粒体积（R2 = 0.66），SIDThr = 39.916+7.843×EE (R2 = 0.41)，SIDTrp = -361.588+4.891×DM+0.387×总淀粉（R2 = 0.85）。