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生长育肥猪饲用高粱的有效能和氨基酸消化率预测。

Prediction of available energy and amino acid digestibility of Chinese sorghum fed to growing-finishing pigs.

机构信息

Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Hunan Research Center of Livestock and Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of Agriculture, National Engineering Laboratory for Poultry Breeding Pollution Control and Resource Technology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.

University of Chinese Academy of Sciences, Beijing 100039, China.

出版信息

J Anim Sci. 2023 Jan 3;101. doi: 10.1093/jas/skad262.

Abstract

Two experiments were conducted to determine digestible energy (DE), metabolizable energy (ME), as well as the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 sorghum samples fed to pigs. In experiment 1, 22 crossbred barrows (Duroc × Yorkshire × Landrace, Initial body weight [BW]: 70.0 ± 1.8 kg) were selected and allotted to a replicated 11 × 3 incomplete Latin square design, including a basal diet and 10 sorghum energy diets and three consecutive periods. Each period had 7 d adaptation and 5 d total feces and urine collection. The DE and ME were determined by the total collection and the difference method. In experiment 2, 22 crossbred barrows (Duroc × Yorkshire × Landrace, Initial BW: 41.3 ± 1.2 kg) that had a T-cannula installed in the distal ileum were assigned to a replicated 11 × 3 incomplete Latin square design, including an N-free diet and 10 sorghum diets. Each period had 5 d adaptation and 2 d ileal digesta collection. The basal endogenous N losses were measured by the N-free diet method. All diets in experiment 2 were added 0.30% titanium dioxide as an indigestible marker for calculating the ileal CP and AA digestibility. On an as-fed basis, the DE and ME contents in sorghum were 3,410 kcal/kg (2,826 to 3,794 kcal/kg) and 3,379 kcal/kg (2,785 to 3,709 kcal/kg), respectively. The best-fit prediction equation for DE and ME were DE = 6,267.945 - (1,271.154 × % tannin) - (1,109.720 × % ash) (R2 = 0.803) and ME = 51.263 + (0.976 × DE) (R2 = 0.994), respectively. The SID of CP, Lys, Met, Thr, and Trp (SIDCP, SIDLys, SIDMet, SIDThr, and SIDTrp) in 10 sorghum samples were 78.48% (69.56% to 84.23%), 74.27% (61.11% to 90.60%), 92.07% (85.16% to 95.40%), 75.46% (66.39% to 80.80%) and 87.99% (84.21% to 92.37%), respectively. The best prediction equations for SID of CP and the first four limiting AAs were as following: SIDCP = 93.404 - (21.026 × % tannin) (R2 = 0.593), SIDCP = 42.922 - (4.011 × % EE) + (151.774 × % Met) (R2 = 0.696), SIDLys = 129.947 - (670.760 × % Trp) (R2 = 0.821), SIDMet = 111.347 - (232.298 × % Trp) (R2 = 0.647), SIDThr = 55.187 + (3.851 × % ADF) (R2 = 0.609) and SIDTrp = 95.676 - (10.824 × % tannin) (R2 = 0.523), respectively. Overall, tannin and ash are the first and second predictors of DE and ME values of sorghum, respectively, and the tannin, EE, Trp, ash, CF, and ADF can be used as the key predictors for SID of CP and first four limiting AAs.

摘要

两个实验旨在确定十种高粱样品的可消化能(DE)、代谢能(ME)以及粗蛋白(CP)和氨基酸(AA)的标准回肠可消化率(SID)。在实验 1 中,选择了 22 头杂交阉猪(杜洛克×约克夏×长白,初始体重[BW]:70.0±1.8kg),并将其分配到一个重复的 11×3 不完全拉丁方设计中,包括一个基础日粮和 10 种高粱能量日粮和三个连续周期。每个周期有 7 天适应期和 5 天总粪便和尿液收集期。DE 和 ME 通过总收集和差值法来确定。在实验 2 中,选择了 22 头杂交阉猪(杜洛克×约克夏×长白,初始 BW:41.3±1.2kg),这些猪的回肠远端安装了 T 型管,然后将其分配到一个重复的 11×3 不完全拉丁方设计中,包括一个无氮日粮和 10 种高粱日粮。每个周期有 5 天适应期和 2 天回肠食糜收集期。基础内源氮损失通过无氮日粮法来测量。实验 2 中的所有日粮均添加了 0.30%的二氧化钛作为不可消化的标记物,用于计算回肠 CP 和 AA 的消化率。基于饲料基础,高粱的 DE 和 ME 含量分别为 3410 千卡/千克(2826 至 3794 千卡/千克)和 3379 千卡/千克(2785 至 3709 千卡/千克)。DE 和 ME 的最佳拟合预测方程分别为 DE=6267.945 -(1271.154×%单宁酸)-(1109.720×%灰分)(R2=0.803)和 ME=51.263+(0.976×DE)(R2=0.994)。10 种高粱样品中 CP、Lys、Met、Thr 和 Trp 的 SID(SIDCP、SIDLys、SIDMet、SIDThr 和 SIDTrp)分别为 78.48%(69.56%至 84.23%)、74.27%(61.11%至 90.60%)、92.07%(85.16%至 95.40%)、75.46%(66.39%至 80.80%)和 87.99%(84.21%至 92.37%)。CP 和前四种限制 AA 的 SID 的最佳预测方程如下:SIDCP=93.404 -(21.026×%单宁酸)(R2=0.593),SIDCP=42.922 -(4.011×%EE)+(151.774×%Met)(R2=0.696),SIDLys=129.947 -(670.760×%Trp)(R2=0.821),SIDMet=111.347 -(232.298×%Trp)(R2=0.647),SIDThr=55.187+(3.851×%ADF)(R2=0.609)和 SIDTrp=95.676 -(10.824×%单宁酸)(R2=0.523)。总体而言,单宁酸和灰分分别是高粱 DE 和 ME 值的第一和第二预测因子,单宁酸、EE、Trp、灰分、CF 和 ADF 可用作 CP 和前四种限制 AA 的 SID 的关键预测因子。

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