A Fresh Look at the Normal Mode Analysis of Proteins: Introducing Allosteric Co-Vibrational Modes.

We propose a new way of utilizing normal modes to study protein conformational transitions. Instead of considering individual modes independently, we show that a weighted mixture of low-frequency vibrational modes can reveal dynamic information about the conformational mechanism in more detail than any single mode can. The weights in the mixed mode, termed the allosteric covibrational mode, are determined using a simple model where the conformational transition is viewed as a perturbation of the coupled harmonic oscillator associated with either of the two conformations. We demonstrate our theory in a biologically relevant example of high pharmaceutical interest involving the V617F mutation of Janus 2 tyrosine kinase (JAK2).