Koch N, Hämmerling G J
Biochemistry. 1985 Oct 22;24(22):6185-90. doi: 10.1021/bi00343a023.
The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further posttranslational maturation of the invariant chain as shown by two-dimensional gel electrophoresis of Ii immunoprecipitates. In particular, the addition of sialic acid residues is blocked. Thus, it appears that fatty acylation is essential for carbohydrate processing of the Ii chain.
小鼠恒定链（Ii）被发现能结合放射性棕榈酸。这种脂肪酸的结合抑制了链间二硫键的形成，可能是因为Ii链跨膜区的半胱氨酸残基被棕榈酰化了。如对Ii免疫沉淀产物进行二维凝胶电泳所示，由浅蓝菌素抑制脂肪酰化会阻断恒定链的进一步翻译后成熟。特别是，唾液酸残基的添加被阻断。因此，脂肪酰化似乎对Ii链的碳水化合物加工至关重要。
