Department of Biology and Plant Ecology, Faculty of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245 Bialystok, Poland.
Department of Evolutionary and Physiological Ecology, Faculty of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245 Bialystok, Poland.
Cells. 2024 Apr 15;13(8):686. doi: 10.3390/cells13080686.
Phytohormones, particularly cytokinin -zeatin (Z), were studied for their impact on the green alga under cadmium (Cd) stress, focusing on growth, metal accumulation, and stress response mechanisms. Using atomic absorption spectroscopy for the Cd level and high-performance liquid chromatography for photosynthetic pigments and phytochelatins, along with spectrophotometry for antioxidants and liquid chromatography-mass spectrometry for phytohormones, we found that Z enhances Cd uptake in , potentially improving phycoremediation of aquatic environments. Cytokinin mitigates Cd toxicity by regulating internal phytohormone levels and activating metal tolerance pathways, increasing phytochelatin synthase activity and phytochelatin accumulation essential for Cd sequestration. Treatment with Z and Cd also resulted in increased cell proliferation, photosynthetic pigment and antioxidant levels, and antioxidant enzyme activities, reducing oxidative stress. This suggests that cytokinin-mediated mechanisms in enhance its capacity for Cd uptake and tolerance, offering promising avenues for more effective aquatic phycoremediation techniques.
植物激素，特别是细胞分裂素-玉米素（Z），在镉（Cd）胁迫下对绿藻的影响进行了研究，重点关注生长、金属积累和应激反应机制。通过原子吸收光谱法测定 Cd 水平、高效液相色谱法测定光合色素和植物螯合肽，以及分光光度法测定抗氧化剂和液相色谱-质谱法测定植物激素，我们发现 Z 增强了 对 Cd 的吸收，可能改善了水生环境的植物修复。细胞分裂素通过调节内部植物激素水平和激活金属耐受途径来减轻 Cd 毒性，增加了植物螯合肽合酶的活性和植物螯合肽的积累，这对于 Cd 的螯合至关重要。Z 和 Cd 的处理还导致细胞增殖、光合色素和抗氧化剂水平以及抗氧化酶活性的增加，从而减轻了氧化应激。这表明， 中的细胞分裂素介导的机制增强了其对 Cd 的吸收和耐受能力，为更有效的水生植物修复技术提供了有前途的途径。
