Burioni Roberto, Silvestrini Lucia, D'Orto Bianca, Tetè Giulia, Nagni Matteo, Polizzi Elisabetta, Gherlone Enrico Felice
Department of Microbiology and Virology, University "Vita-Salute" San Raffaele, 20132 Milan, Italy.
Department of Dentistry, Dental School, IRCCS San Raffaele Hospital, "Vita-Salute" San Raffaele University, 20132 Milan, Italy.
Bioengineering (Basel). 2024 Apr 16;11(4):387. doi: 10.3390/bioengineering11040387.
The objective of this in vitro study was to compare reused and sterilized versus new healing abutments to assess whether a decontamination and sterilization process performed on resued healing abutments was sufficient to remove residual proteins. The two groups were comparable with respect to patient safety.
During the period from September 2022 to October 2023, healing abutment screws were selected and divided into two groups according to whether they were new or previously used in patients. The samples were subjected to a decontamination and sterilization protocol, and results from sample sterility evaluation and assessment of surface protein levels were recorded.
The obtained results revealed a significant difference in the OD562 nm values between new and reused healing abutment samples. The assay demonstrates how treated healing abutments were still contaminated by residual proteins.
Within the limitations of the present study, although from an infectious point of view sterilization results in the total eradication of pathogens, surface proteins remain on reused healing abutments.
本体外研究的目的是比较重复使用并灭菌的愈合基台与新的愈合基台，以评估对重复使用的愈合基台进行去污和灭菌处理是否足以去除残留蛋白质。两组在患者安全性方面具有可比性。
在2022年9月至2023年10月期间，选择愈合基台螺钉，并根据其是新的还是先前在患者中使用过分为两组。对样本进行去污和灭菌方案处理，并记录样本无菌评估结果和表面蛋白水平评估结果。
获得的结果显示，新的和重复使用的愈合基台样本之间在OD562 nm值上存在显著差异。该检测表明经处理的愈合基台仍被残留蛋白质污染。
在本研究的局限性范围内，尽管从感染角度来看灭菌可导致病原体的完全根除，但重复使用的愈合基台上仍残留有表面蛋白。
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