Bueno Manuela R, Martins Fernando H, Rocha Catarina M, Kawamoto Dione, Ishikawa Karin H, Ando-Suguimoto Ellen S, Carlucci Aline R, Arroteia Leticia S, Casarin Renato V, Mayer Marcia P A
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, SP, Brazil.
Department of Periodontology, Faculdade São Leopoldo Mandic, Campinas 13045-755, SP, Brazil.
Microorganisms. 2024 Apr 22;12(4):836. doi: 10.3390/microorganisms12040836.
The benefits of probiotics on dysbiotic microbiomes and inflammation are dependent on the tested strain, host factors, and the resident microbiome. There is limited knowledge on the effects of probiotics in -associated periodontitis. Thus, LA5 (LA5) was orally inoculated for 30 days in C57Bl/6 mice infected with JP2 (Aa) and (Sg). Alveolar bone loss, gingival gene expression, and oral and gut microbiomes were determined. LA5 controlled bone loss in Aa+Sg-infected mice, downregulated the expression of and upregulated in gingival tissues, and altered the oral and gut microbiomes. LA5 increased the diversity of the oral microbiome of Aa+Sg infected mice, and Aa+Sg and Aa+Sg+LA5 oral or gut microbiomes clustered apart. LA5 induced shifts in Aa+Sg infected mice by increasing the abundance of and decreasing in the oral cavity and increasing the abundance of and in the gut. In conclusion, LA5 oral administration controls experimental Aa-associated periodontitis by altering inflammatory gene expression and the oral and gut microbiomes.
益生菌对失调微生物群和炎症的益处取决于所测试的菌株、宿主因素和常驻微生物群。关于益生菌在伴放线聚集杆菌（Aa）相关性牙周炎中的作用，目前了解有限。因此，在感染了Aa JP2和牙龈卟啉单胞菌（Pg）的C57Bl/6小鼠中口服接种嗜酸乳杆菌LA5（LA5）30天。测定牙槽骨吸收、牙龈基因表达以及口腔和肠道微生物群。LA5可控制Aa+Pg感染小鼠的骨吸收，下调牙龈组织中肿瘤坏死因子α（TNF-α）的表达并上调白细胞介素-10（IL-10）的表达，还可改变口腔和肠道微生物群。LA5增加了Aa+Pg感染小鼠口腔微生物群的多样性，且Aa+Pg和Aa+Pg+LA5的口腔或肠道微生物群聚类不同。LA5通过增加口腔中血链球菌和轻链球菌的丰度以及降低具核梭杆菌的丰度，并增加肠道中双歧杆菌属和乳杆菌属的丰度，从而在Aa+Pg感染小鼠中诱导菌群变化。总之，口服LA5可通过改变炎症基因表达以及口腔和肠道微生物群来控制实验性Aa相关性牙周炎。
