School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Dalian Probiotics Function Research Key Laboratory, Dalian Polytechnic University, Dalian 116034, China.
Nutrients. 2022 May 3;14(9):1916. doi: 10.3390/nu14091916.
In our previous research, Lactiplantibacillus plantarum-12 alleviated inflammation in dextran sodium sulfate (DSS)-induced mice by regulating intestinal microbiota and preventing colon shortening (p < 0.05). The purpose of the present study was to evaluate whether L. plantarum-12 could ameliorate the colon cancer symptoms of azoxymethane (AOM)/DSS-treated C57BL/6 mice. The results showed that L. plantarum-12 alleviated colonic shortening (from 7.43 ± 0.15 to 8.23 ± 0.25) and weight loss (from 25.92 ± 0.21 to 27.75 ± 0.88) in AOM/DSS-treated mice. L. plantarum-12 oral administration down-regulated pro-inflammatory factors TNF-α (from 350.41 ± 15.80 to 247.72 ± 21.91), IL-8 (from 322.19 ± 11.83 to 226.08 ± 22.06), and IL-1β (111.43 ± 8.14 to 56.90 ± 2.70) levels and up-regulated anti-inflammatory factor IL-10 (from 126.08 ± 24.92 to 275.89 ± 21.87) level of AOM/DSS-treated mice. L. plantarum-12 oral administration restored the intestinal microbiota dysbiosis of the AOM/DSS treated mice by up-regulating beneficial Muribaculaceae, Lactobacillaceae, and Bifidobacteriaceae levels and down-regulating pathogenic Proteobacteria, Desulfovibrionaceae, and Erysipelotrichaceae levels. As a result, the fecal metabolites of the AOM/DSS-treated mice were altered, including xanthosine, uridine, 3,4-methylenesebacic acid, 3-hydroxytetradecanedioic acid, 4-hydroxyhexanoylglycine, beta-leucine, and glycitein, by L. plantarum-12 oral administration. Furthermore, L. plantarum-12 oral administration significantly ameliorated the colon injury of the AOM/DSS-treated mice by enhancing colonic tight junction protein level and promoting tumor cells death via down-regulating PCNA (proliferating cell nuclear antigen) and up-regulating pro-apoptotic Bax. (p < 0.05). Taken together, L. plantarum-12 oral administration could ameliorate the colon cancer burden and inflammation of AOM-DSS-treated C57BL/6 mice through regulating the intestinal microbiota, manipulating fecal metabolites, enhancing colon barrier function, and inhibiting NF-κB signaling. These results suggest that L. plantarum-12 might be an excellent probiotic candidate for the prevention of colon cancer.
在我们之前的研究中，植物乳杆菌 -12 通过调节肠道微生物群和防止结肠缩短来减轻葡聚糖硫酸钠（DSS）诱导的小鼠的炎症（p<0.05）。本研究旨在评估植物乳杆菌 -12 是否可以改善氧化偶氮甲烷（AOM）/DSS 处理的 C57BL/6 小鼠的结肠癌症状。结果表明，植物乳杆菌 -12 减轻了 AOM/DSS 处理小鼠的结肠缩短（从 7.43±0.15 至 8.23±0.25）和体重减轻（从 25.92±0.21 至 27.75±0.88）。植物乳杆菌 -12 口服给药下调促炎因子 TNF-α（从 350.41±15.80 至 247.72±21.91）、IL-8（从 322.19±11.83 至 226.08±22.06）和 IL-1β（111.43±8.14 至 56.90±2.70）水平，并上调抗炎因子 IL-10（从 126.08±24.92 至 275.89±21.87）水平。植物乳杆菌 -12 口服给药通过上调有益的 Muribaculaceae、Lactobacillaceae 和 Bifidobacteriaceae 水平和下调致病的 Proteobacteria、Desulfovibrionaceae 和 Erysipelotrichaceae 水平，恢复了 AOM/DSS 处理小鼠的肠道微生物失调。结果，AOM/DSS 处理小鼠的粪便代谢物发生变化，包括黄嘌呤核苷、尿苷、3,4-亚甲基癸二酸、3-羟基十四烷二酸、4-羟基己酰甘氨酸、β-亮氨酸和大豆苷元，这是通过植物乳杆菌 -12 口服给药实现的。此外，植物乳杆菌 -12 口服给药通过增强结肠紧密连接蛋白水平和通过下调 PCNA（增殖细胞核抗原）和上调促凋亡 Bax 来促进肿瘤细胞死亡，显著改善了 AOM/DSS 处理小鼠的结肠损伤（p<0.05）。综上所述，植物乳杆菌 -12 通过调节肠道微生物群、操纵粪便代谢物、增强结肠屏障功能和抑制 NF-κB 信号通路，可减轻 AOM-DSS 处理的 C57BL/6 小鼠的结肠癌负担和炎症。这些结果表明，植物乳杆菌 -12 可能是预防结肠癌的优秀益生菌候选物。
