Zhang Xinping, Ma Yuan, Wang Yujiao, Liang Zhenwei, Zhang Xuanhe, Chen Yiqing, Wang Qingyi, Qin Hua, Wang Jiadao
School of Mechanical-Electronic and Vehicle Engineering, Beijing University of Civil Engineering and Architecture, Beijing 102616, China.
Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Micromachines (Basel). 2024 Apr 18;15(4):542. doi: 10.3390/mi15040542.
The heterogeneity of circulating tumor cells has a significant impact on the diagnosis, treatment, and monitoring of cancer. Research on the subtypes of circulating tumor cells can bring better treatment outcomes for cancer patients. Here, we proposed a microfluidic chip for the magnetic capture of subtypes of circulating tumor cells from the whole blood and phenotypic profiling by stacking laminar flow vertically. Circulating tumor cells were sorted and captured by the three-dimensional regulation of both magnetic fields in the vertical direction and flow fields in the lateral direction. Using EpCAM-magnetic beads, we achieved sorting and sectional capture of target cells in whole blood and analyzed the surface expression levels of the captured cells, confirming the functionality of the microfluidic chip in sorting and capturing subtypes of circulating tumor cells. This microfluidic chip can also aid in the subsequent subtype analysis of other rare cells.
循环肿瘤细胞的异质性对癌症的诊断、治疗和监测具有重大影响。对循环肿瘤细胞亚型的研究可为癌症患者带来更好的治疗效果。在此，我们提出了一种微流控芯片，用于从全血中磁性捕获循环肿瘤细胞亚型，并通过垂直堆叠层流进行表型分析。通过垂直方向磁场和横向流场的三维调控对循环肿瘤细胞进行分选和捕获。利用EpCAM磁珠，我们实现了全血中靶细胞的分选和分段捕获，并分析了捕获细胞的表面表达水平，证实了该微流控芯片在分选和捕获循环肿瘤细胞亚型方面的功能。这种微流控芯片还可辅助后续对其他稀有细胞的亚型分析。
