Elekhtiar Sally A, Abo Gazia Maha Mohamed, Helal Azza I, Mahfouz Hala, El-Kemary Nesma M, Abd-Elsalam Sherief, Elksass Samar, Alkabes Hend A, El-Kemary Maged, Abd-Elsalam Marwa M
Department of Histology, Faculty of Medicine, Kafrelsheikh University, Kafr ElSheikh, Egypt.
Department of Histology, Faculty of Medicine, New Mansoura University, Dakahlia, Egypt.
Endocr Metab Immune Disord Drug Targets. 2024 Apr 26. doi: 10.2174/0118715303280532240415094718.
Diabetic wound represents a serious issue with a substantial impact and an exceptionally complex pathology affecting patients' mental health and quality of life. So, we have developed a novel 3D organo-hydrogel nanocomposite of polydopamine/TiO2 nanoparticles and cu (PDA-TiO2@Cu) and examined its efficacy in diabetic wound healing.
Forty-five adult male albino rats were divided into normal control rats (non-diabetic rats with non-treated skin wounds), diabetic control rats (diabetic rats with non-treated skin wounds), and organo-hydrogel-treated rats (diabetic wounds treated with topically applied organo- hydrogel once daily). Macroscopic changes of the wound were observed on days 0, 3, 5, 7, and 10 to measure wound diameters. Skin specimens from the wound tissue were taken on days 3, 7, and 10, respectively, and examined histologically and immunohistochemically. Also, the gene expressions of collagen I, Matrix Metalloproteinase-9 (MMP-9), and Epidermal Growth Factor (EGF), and levels of Interleukin 6 (IL-6) and Superoxide Dismutase (SOD) were assessed.
Our observed results indicated that the developed patch significantly accelerated the healing time compared to the normal control and diabetic control groups. Moreover, the patchloaded group revealed complete re-epithelization and a highly significant increase in the mean area % of CD31 immunostaining on day 7. The organo-hydrogel-loaded group displayed a significant decrease in gene expression of MMP-9 and a significant increase in gene expression of EGF and collagen I. Additionally, the organo-hydrogel-loaded group exhibited a significant decrease in levels of IL-6 and a significant increase in levels of SOD, compared to the normal diabetic control groups.
The organo-hydrogel can be used for treating and decreasing the healing period of diabetic wounds.
糖尿病伤口是一个严重问题，具有重大影响且病理异常复杂，会影响患者的心理健康和生活质量。因此，我们研发了一种新型的聚多巴胺/TiO₂纳米颗粒与铜的三维有机水凝胶纳米复合材料（PDA-TiO₂@Cu），并研究了其在糖尿病伤口愈合中的疗效。
将45只成年雄性白化大鼠分为正常对照大鼠（非糖尿病大鼠，皮肤伤口未处理）、糖尿病对照大鼠（糖尿病大鼠，皮肤伤口未处理）和有机水凝胶处理大鼠（糖尿病伤口，每天局部应用有机水凝胶一次）。在第0、3、5、7和10天观察伤口的宏观变化以测量伤口直径。分别在第3、7和10天从伤口组织采集皮肤标本，并进行组织学和免疫组织化学检查。此外，评估胶原蛋白I、基质金属蛋白酶-9（MMP-9）和表皮生长因子（EGF）的基因表达，以及白细胞介素6（IL-6）和超氧化物歧化酶（SOD）的水平。
我们的观察结果表明，与正常对照组和糖尿病对照组相比，所研发的贴片显著缩短了愈合时间。此外，贴片加载组在第7天显示完全重新上皮化，且CD31免疫染色的平均面积百分比显著增加。与正常糖尿病对照组相比，有机水凝胶加载组MMP-9的基因表达显著降低，EGF和胶原蛋白I的基因表达显著增加。此外，有机水凝胶加载组IL-6水平显著降低，SOD水平显著增加。
有机水凝胶可用于治疗糖尿病伤口并缩短愈合期。
