Xu Hui, Cai Yu, Yang Hua, Li Shanglai, Chen Peiyong, Wei Zongyou, Wang Feng, Wang Ziyu, Zhang Yanli
Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China.
Taicang Animal husbandry and veterinary station, Taicang 215400, China.
Anim Reprod Sci. 2024 Jun;265:107457. doi: 10.1016/j.anireprosci.2024.107457. Epub 2024 Mar 13.
The anterior pituitary plays a critical role in the endocrine system, contains gonadotrophs, which regulate reproductive efficiency by secreting follicle-stimulating hormone (FSH) and luteinizing hormone (LH). PPP2R2A is a serine-threonine phosphatase that regulates reproductive functions in both females and males, its function in pituitary cells remain unclear. Hu sheep is a highly prolific breed, which makes it suitable for studying reproductive mechanisms. In this study, the relative abundances of PPP2R2A mRNA expression were higher in the pituitary of high-prolificacy (HF) Hu sheep compared to those of low-prolificacy (LF) Hu sheep. Additionally, we demonstrated that PPP2R2A promotes pituitary cell proliferation and gonadotropin secretion using the EdU assay and ELISA, respectively. Moreover, it inhibits pituitary cell apoptosis using flow cytometry. Furthermore, PPP2R2A may affect pituitary cell function by regulating the AKT/mTOR signaling pathway. In summary, our findings suggest that PPP2R2A may play a role in regulating pituitary function and influencing the secretion of gonadotropins.
腺垂体在内分泌系统中发挥着关键作用，其中含有促性腺细胞，这些细胞通过分泌促卵泡激素（FSH）和黄体生成素（LH）来调节生殖效率。PPP2R2A是一种丝氨酸 - 苏氨酸磷酸酶，可调节雌性和雄性的生殖功能，但其在垂体细胞中的功能仍不清楚。湖羊是一种高繁殖力品种，这使其适合用于研究生殖机制。在本研究中，与低繁殖力（LF）湖羊相比，高繁殖力（HF）湖羊垂体中PPP2R2A mRNA表达的相对丰度更高。此外，我们分别使用EdU检测法和ELISA证明了PPP2R2A促进垂体细胞增殖和促性腺激素分泌。此外，它通过流式细胞术抑制垂体细胞凋亡。此外，PPP2R2A可能通过调节AKT/mTOR信号通路影响垂体细胞功能。总之，我们的研究结果表明，PPP2R2A可能在调节垂体功能和影响促性腺激素分泌中发挥作用。
