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刺突蛋白 RBD 中的糖基化位点降低了 PEDV AH2012/12 的免疫原性。

The glycosylation sites in RBD of spike protein attenuate the immunogenicity of PEDV AH2012/12.

机构信息

College of Animal Science, Yangtze University, Jingzhou 434025, China; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, Jiangsu 210014, China.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, Jiangsu 210014, China.

出版信息

Virus Res. 2024 Jul;345:199381. doi: 10.1016/j.virusres.2024.199381. Epub 2024 May 2.

DOI:10.1016/j.virusres.2024.199381
PMID:38679392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11070342/
Abstract

Porcine epidemic diarrhea (PED) is a highly contagious swine intestinal disease caused by PED virus (PEDV). Vaccination is a promising strategy to prevent and control PED. Previous studies have confirmed that glycosylation could regulate the immunogenicity of viral antigens. In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV, we found that removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in RBD is a promising method to develop PEDV vaccines.

摘要

猪流行性腹泻（PED）是由猪流行性腹泻病毒（PEDV）引起的一种高度传染性猪肠道疾病。疫苗接种是预防和控制 PED 的一种有前途的策略。先前的研究已经证实，糖基化可以调节病毒抗原的免疫原性。在本研究中，我们构建了三个去除 RBD 中糖基化位点的重组 PEDV。病毒感染实验表明，重组病毒与亲本 PEDV 具有相似的复制特征。尽管动物攻毒研究表明 RBD 中的糖基化位点不影响 PEDV 的致病性，但我们发现去除 RBD 区域的糖基化位点可以促进体内 IgG 和中和滴度的提高，表明 RBD 中的去糖基化可以增强 PEDV 的免疫原性。这些发现表明，去除 RBD 中的糖基化位点是开发 PEDV 疫苗的一种有前途的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/dc0919efeaf4/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/1aeeeba500d1/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/0358b22c678a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/819ad7b747b3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/7c29305b04b2/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/dc0919efeaf4/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/1aeeeba500d1/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/0358b22c678a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/819ad7b747b3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/7c29305b04b2/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b93/11070342/dc0919efeaf4/gr5.jpg

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